Mycophenolate mofetil improves renal haemodynamics, microvascular oxygenation, and inflammation in a rat model of supra-renal aortic clamping-mediated renal ischaemia reperfusion injury
Mycophenolate mofetil improves renal haemodynamics, microvascular oxygenation, and inflammation in a rat model of supra-renal aortic clamping-mediated renal ischaemia reperfusion injury
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DOI：
10.1111/1440-1681.12687
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发表时间：
2017-02-01
影响因子：
2.9
通讯作者：
Ince, Can
中科院分区：
文献类型：
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作者：
Ergin, Bulent;Heger, Michal;Ince, Can
缺血再灌注(I/R)是急性肾损伤(AKI)的主要原因之一，以无菌炎症和氧化应激为特征。免疫细胞激活可引起炎症介质和活性氧物种(ROS)的过度产生，导致局部和远程组织损伤相关的微循环和组织氧合紊乱。本研究探讨了临床使用的免疫抑制剂霉酚酸酯(MMF)是否能够通过预防无菌炎症来减少I/R引起的肾脏氧合缺陷和氧化应激。将大鼠分为3组(n=6/组)：(1)假手术对照组；(2)单纯肾I/R组(I/R)；(3)I/R+MMF治疗组(I/R前20 mg/kg)。在腹主动脉上放置血管封堵器30分钟，然后再灌流120分钟，造成缺血。肾I/R加重肾氧合作用(P
Ischaemia/reperfusion (I/R) is one of the main causes of acute kidney injury (AKI), which is characterized by sterile inflammation and oxidative stress. Immune cell activation can provoke overproduction of inflammatory mediators and reactive oxygen species (ROS), leading to perturbation of the microcirculation and tissue oxygenation associated with local and remote tissue injury. This study investigated whether the clinically employed immunosuppressant mycophenolate mofetil (MMF) was able to reduce I/R-induced renal oxygenation defects and oxidative stress by preventing sterile inflammation. Rats were divided into three groups (n=6/group): (1) a sham-operated control group; (2) a group subjected to renal I/R alone (I/R); and (3) a group subjected to I/R and MMF treatment (20 mg/kg prior to I/R) (I/R+MMF). Ischaemia was induced by a vascular occluder placed on the abdominal aorta for 30 minutes, followed by 120 minutes of reperfusion. Renal I/R deteriorated renal oxygenation (P