Immunohistochemical study of human pterygium.
Immunohistochemical study of human pterygium.
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人翼状胬肉的免疫组织化学研究。
DOI：
10.14670/hh-17.139
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发表时间：
2002
影响因子：
2
通讯作者：
P. Sirigu
中科院分区：
文献类型：
--
作者：
M. Perra;C. Maxia;I. Zucca;F. Piras;P. Sirigu
本研究的目的是评估人类翼状胬肉组织的免疫组织化学特征，以确定翼状胬肉发病机制中免疫机制的可能贡献，并研究翼状胬肉组织中某些黑色素瘤相关抗原的存在，以评估这两种疾病是否存在相关性。通过手术切除翼状胬肉获得人类翼状胬肉活检标本。将组织切片固定并处理用于石蜡包埋。使用抗生物素蛋白-生物素过氧化物酶法或链霉抗生物素蛋白-碱性磷酸酶法对切片进行免疫组织化学染色，以显示伊加、IgM、IgG、CD 3、CD 20、CD 68、HLA-DR、蛋白S100、HMB 45和Melan A。研究结果表明，粘膜免疫系统的所有效应成分都存在于人类翼状胬肉中，并且在黑色素瘤最敏感的标志物中，只有S100显示出免疫反应性。免疫发病机制似乎是翼状胬肉发病机制的原因，可能是由预先存在的结膜炎或微创伤与患者的易感性相结合引起的。翼状胬肉与黑色素瘤之间无相关性。
The purpose of this study has been to evaluate the immunohistochemical characteristics of human pterygial tissues in order to ascertain the possible contribution of an immunological mechanism in the pathogenesis of pterygium and to investigate the presence in the pterygial tissues of some melanoma-associated antigens, in order to evaluate if there may be a small possibility of correlation of the two diseases. Human biopsy specimens of pterygium were obtained by surgery for pterygium excision. Tissue segments were fixed and processed for paraffin embedding. Microtome sections were treated for the immunohistochemical demonstration of IgA, IgM, IgG, CD3, CD20, CD68, HLA-DR, Protein S100, HMB45, and Melan A using the avidin-biotin peroxidase method or the streptavidin biotin-alkaline phosphatase method. The findings suggest that all the effector components of the mucosal immune system are present in the human pterygium and, among the most sensitive markers for melanoma, only S100 shows immunoreactivity. An immunopathogenetic mechanism seems to be responsible for the pathogenesis of pterygium, perhaps being caused by pre-existing conjunctivitis or microtrauma in combination with the patient's predisposition. No correlation between pterygium and melanoma was found.