CELL-DEATH ORGANIZES THE POSTNATAL-DEVELOPMENT OF THE TRIGEMINAL INNERVATION OF THE CEREBRAL VASCULATURE
CELL-DEATH ORGANIZES THE POSTNATAL-DEVELOPMENT OF THE TRIGEMINAL INNERVATION OF THE CEREBRAL VASCULATURE
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DOI：
10.1016/0165-3806(86)90248-8
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发表时间：
1986-06-01
期刊：
影响因子：
--
通讯作者：
VANDERKOOY, D
中科院分区：
文献类型：
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作者：
OCONNOR, TP;VANDERKOOY, D
在成人三叉神经节中，支配大脑中动脉（MCA）的单细胞体与支配前额的一个或多个细胞体不同，但位置接近（O''Connor 和 Van der Kooy，提交）。采用出生后第 3-90 天大鼠的多重荧光逆行轴突追踪来描述这种成人三叉神经投射模式的发展。我们发现，在出生后第 5 天 (PND 5)，近 90% 的神经支配 MCA 的细胞被 PND 90 消除。在出生后的同一时期，支配前额的神经节细胞只有不到 20% 死亡。观察到神经节细胞亚群在出生后不同时期具有最高死亡率。首先，整个眼科分裂中的 15-20% 细胞在 PND 5 和 PND 10 之间死亡。其次，一小群早期投射到对侧 MCA 的细胞在 PND 22 时完全消失。第三，仅投射到 MCA 的细胞主要在 PND 10 和 PND 54 之间死亡。第四，在产后第一周，有许多细胞投射到 MCA 和前额；然而，该群体中的 90% 会在 PND 90 时死亡。这种消除是在出生后最迟观察到的，这些细胞在 PND 22 和 PND 90 之间表现出最高的细胞死亡率。因此，细胞死亡是在三叉神经节眼部分裂中产生这种组织的主要出生后机制，而轴突侧枝的回缩是次要机制。我们认为，具有分叉动脉和前额投射的细胞的最新死亡期以及一些超过PND 90的动脉投射细胞的最终持续存在，可能是由于这些三叉神经节细胞的周围神经支配区域更大。
In the adult trigeminal ganglion single cell bodies that innervate the middle cerebral artery (MCA) are different from but situated near to one or more cell bodies that innervate the forehead (O''Connor and Van der Kooy, submitted). Multiple fluorescent retrograde axonal tracing in postnatal day 3-90 rats was employed to describe the development of this adult pattern of trigeminal projections. We found that close to 90% of the cells that innervate the MCA at postnatal day 5 (PND 5) are eliminated by PND 90. Less than 20% of the ganglion cells innervating the forehead die over the same postnatal period. Subpopulations of cells in the ganglion were observed to have a maximal rate of death during different postnatal periods. First, 15-20% of the cells throughout the ophthalmic division die between PND 5 and PND 10. Second, a small population of cells that had early projections to the contralateral MCA die out completely by PND 22. Third, cells with a projection only to the MCA die primarily between PND 10 and PND 54. Fourth, during the first post-natal week there are many cells that project to both the MCA and the forehead; however, 90% of this population dies by PND 90. This elimination is observed latest in the postnatal period, with these cells exhibiting their greatest rate of cell death between PND 22 and PND 90. Thus, cell death is the primary postnatal mechanism that produces this organization in the ophthalmic division of the trigeminal ganglion and retraction of axonal collaterals is a minor mechanism. We suggest that the latest period of death in cells with divergent artery and forehead projections as well as the ultimate persistence of some artery projecting cells beyond PND 90, may be due to the larger peripheral fields of innervation of these trigeminal ganglion cells.