The effect of platelet-rich plasma on the cellular response of rat bone marrow cells in vitro
The effect of platelet-rich plasma on the cellular response of rat bone marrow cells in vitro
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DOI：
10.1016/j.tripleo.2005.03.007
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发表时间：
2005-09-01
影响因子：
--
通讯作者：
Koyano, K
中科院分区：
文献类型：
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作者：
Ogino, Y;Ayukawa, Y;Koyano, K
本研究旨在探讨富血小板血浆（PRP）对大鼠骨髓细胞（RBMCs）增殖和分化的影响。PRP、贫血小板血浆（PPP）和骨髓细胞来源于大鼠（心脏和胫骨），并在有或没有PRP或PPP的情况下培养细胞（0 [对照]，0.2类似于10 μ L/mL）。在第2天和第4天测量RBC的增殖，并在第4天和第8天评价碱性磷酸酶（ALP）染色和活性测量以确定PRP对分化的影响。与对照组相比，PRP显著促进细胞增殖（P < .05）。这些增强作用大于由添加PPP引起的增强作用。ALP染色显示PRP使ALP阳性细胞数和ALP活性显著降低（P <0.05）。我们的结果表明，PRP刺激红细胞的增殖，但抑制分化。
The aim of this study was to investigate the effect of platelet-rich plasma (PRP) on the proliferation and the differentiation of rat bone marrow cells (RBMCs). PRP, platelet-poor plasma (PPP), and bone marrow cells were derived from the rats (hearts and tibia) and the cells were cultured with or without PRP or PPP (0 [control]), 0.2 similar to 10 mu L/mL). The proliferation of RBMCs was measured on days 2 and 4, and alkaline phosphatase (ALP) staining and activity measurement were evaluated to determine the effect of PRP on the differentiation on days 4 and 8. PRP enhanced the proliferation significantly compared to the control group (P < .05). These enhancements were greater than ones induced by the addition of PPP. ALP staining appeared to show that PRP decreased the number of ALP positive cells and ALP activity significantly (P < .05). Our results demonstrate that PRP stimulates the proliferation but suppresses the differentiation of RBMCs.