Differential proteomic analysis of actinic keratosis, Bowen's disease and cutaneous squamous cell carcinoma by label-free LC-MS/MS
Differential proteomic analysis of actinic keratosis, Bowen's disease and cutaneous squamous cell carcinoma by label-free LC-MS/MS
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DOI：
10.1016/j.jdermsci.2018.04.006
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发表时间：
2018-07-01
影响因子：
4.6
通讯作者：
Fernandez-Penas, Pablo
中科院分区：
文献类型：
--
作者：
Azimi, Ali;Kaufman, Kimberley L.;Fernandez-Penas, Pablo
背景：光化性角化病(AK)、鲍温病(BD)和皮肤鳞状细胞癌(CSCC)之间的界限有时并不清楚。目的：评价正常表皮、AK、BD和CSCC之间的蛋白质组学变化，以支持分子分类，提高对疾病进展的认识。方法：对4例正常表皮、10例AK、10例BD和10例CSCC进行显微解剖石蜡包埋标本的质谱分析。在归一化和多次测试调整之后，使用微阵列数据的线性模型进行差异丰度分析。对蛋白质进行有意义的过滤(调整p值
Background: The boundaries between actinic keratosis (AK), Bowen's disease (BD), and cutaneous squamous cell carcinoma (cSCC) are sometimes not clear. Large-scale proteomic profiling studies of these lesions are also non-existent.Objective: To evaluate proteomic changes between normal epidermis, AK, BD and cSCC that could support a molecular classification and improve our understanding of disease progression.Methods: Microdissected formalin-fixed paraffin embedded samples of normal epidermis (n = 4, pooled), AK (n = 10), BD (n = 10) and cSCC (n = 10) were analyzed by mass spectrometry. Following normalization and multiple testing adjustments, differential abundance analysis was performed using Linear Models for Microarray data. Proteins were filtered for significance (adjusted p-value