A comparison of the reproductive physiology of largemouth bass, Micropterus salmoides, collected from the Escambia and Blackwater Rivers in Florida
A comparison of the reproductive physiology of largemouth bass, Micropterus salmoides, collected from the Escambia and Blackwater Rivers in Florida
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DOI：
10.2307/3434509
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发表时间：
1999-03-01
影响因子：
10.4
通讯作者：
Guillette, LJ
中科院分区：
文献类型：
--
作者：
Orlando, EF;Denslow, ND;Guillette, LJ
大口黑鲈 (LMB)（Micropterus salmoides）采自佛罗里达州彭萨科拉附近的埃斯坎比亚河（污染地点）和黑水河（参考地点）。埃斯坎比亚河的收集发生在两个已确定的污染点源的废水下游。这些点源包括一家燃煤发电厂和一家化工公司。相反，黑水河的源头及其大部分长度都在凝视公园内流动。尽管黑水河的下游有一些开发，但在更原始的上游区域收集了鱼类。通过电击捕获鱼并将其保存在通风冷却器中。进行身体测量，抽取血液，并切除肝脏和性腺。使用经过验证的放射免疫测定法测定 LMB 血浆中 17 β-雌二醇 (E-2) 和睾酮的浓度。使用针对大口黑鲈卵黄蛋白原进行验证的单克隆抗体，通过凝胶电泳 (SDS-PAGE) 和蛋白质印迹法测定卵黄蛋白原的存在。在两个地点的雌性中未观察到 E-2 或睾酮血浆浓度存在差异。同样，男性的血浆 E-2 也没有表现出差异。然而，与参考地点相比，来自污染地点的男性的血浆睾酮水平较低。产生卵黄的雄性仅发生在污染地点。此外，与参考地点的男性相比，来自污染地点的男性的肝脏质量比例更高。这些数据表明，肝酶活性的增加可能改变了生殖类固醇水平，并且卵黄雄性的存在表明埃斯坎比亚河中存在外源雌激素。
Largemouth bass (LMB), Micropterus salmoides, were taken from the Escambia River (contaminated site) and the Blackwater River (reference site) near Pensacola, Florida. The Escambia River collection occurred downstream of the effluent from two identified point sources of pollution. These point sources included a coal-fired electric power plant and a chemical company. Conversely, the Blackwater River's headwaters and most of its length flow within a stare park Although there is some development on the lower part of the Blackwater River, fish were collected in the more pristine upper regions. Fish were captured by electroshocking and were maintained in aerated coolers. Physical measurements were obtained, blood was taken, and liver and gonads were removed. LMB plasma was assayed for the concentration of 17 beta-estradiol (E-2) and testosterone using validated radioimmunoassays. The presence of vitellogenin was determined by gel electrophoresis (SDS-PAGE) and Western blotting using a monoclonal antibody validated for largemouth bass vitellogenin. No differences in plasma concentrations of E-2 or testosterone were observed in females from the two sites. Similarly, males exhibited no difference in plasma E-2. However, plasma testosterone was lower in the males from the contaminated site, as compared to the reference site. Vitellogenic males occurred only at the contaminated site. Additionally, liver mass was proportionately higher in males from the contaminated site, as compared to males from the reference site. These data suggest that reproductive steroid levels may have been altered by increased hepatic enzyme activity, and the presence of vitellogenic males indicates that an exogenous source of estrogen was present in the Escambia River.