Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth.
Tomato Seed Coat Permeability to Selected Carbon Nanomaterials and Enhancement of Germination and Seedling Growth.
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DOI：
10.1155/2015/419215
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发表时间：
2015
影响因子：
--
通讯作者：
Taylor AG
中科院分区：
文献类型：
--
作者：
Ratnikova TA;Podila R;Rao AM;Taylor AG
使用模型检查种皮渗透性，该模型测试了浸泡番茄（Solanum lycopersicon）种子与碳基纳米材料（CBNM）和超声波照射（美国）相结合的效果。研究了 CBNM 对种皮的渗透，以及 CBNM 对种子萌发和幼苗生长的影响。两种 CBNM，C60(OH)20（富勒醇）和多壁纳米管 (MWNT)，以 50 mg/L 的浓度施用，处理时间为 0 至 60 分钟。明场、荧光、电子显微镜和显微拉曼光谱提供了确凿的证据，证明这两种 CBNM 都无法穿透种皮。纳米材料（NM）吸收的限制归因于位于邻近胚乳的种皮最内层的半透层。在 MWNT 存在下使用 US 进行 30 或 60 分钟的种子处理会物理破坏种皮；然而，半透层的完整性并未受到损害。 MWNT 存在时，发芽率、幼苗长度和重量均有所提高，但 C60(OH)20 并未改变。种子同时接触纳米材料和超声可以深入了解纳米颗粒与种子的相互作用，并可作为增强种子萌发和早期幼苗生长的递送系统。
Seed coat permeability was examined using a model that tested the effects of soaking tomato (Solanum lycopersicon) seeds in combination with carbon-based nanomaterials (CBNMs) and ultrasonic irradiation (US). Penetration of seed coats to the embryo by CBNMs, as well as CBNMs effects on seed germination and seedling growth, was examined. Two CBNMs, C60(OH)20 (fullerol) and multiwalled nanotubes (MWNTs), were applied at 50 mg/L, and treatment exposure ranged from 0 to 60 minutes. Bright field, fluorescence, and electron microscopy and micro-Raman spectroscopy provided corroborating evidence that neither CBNM was able to penetrate the seed coat. The restriction of nanomaterial (NM) uptake was attributed to the semipermeable layer located at the innermost layer of the seed coat adjacent to the endosperm. Seed treatments using US at 30 or 60 minutes in the presence of MWNTs physically disrupted the seed coat; however, the integrity of the semipermeable layer was not impaired. The germination percentage and seedling length and weight were enhanced in the presence of MWNTs but were not altered by C60(OH)20. The combined exposure of seeds to NMs and US provided insight into the nanoparticle-seed interaction and may serve as a delivery system for enhancing seed germination and early seedling growth.