Early dissociation of protein synthesis and amylase secretion following hormonal stimulation of the pancreas.
Early dissociation of protein synthesis and amylase secretion following hormonal stimulation of the pancreas.
复制标题
胰腺激素刺激后蛋白质合成和淀粉酶分泌的早期分离。
DOI：
10.1139/y74-027
复制
发表时间：
1974
影响因子：
2.1
通讯作者：
J. Morisset
中科院分区：
文献类型：
--
作者：
R. Mongeau;Y. Couture;J. Dunnigan;J. Morisset
激素和胆碱能刺激可增加各种胰腺酶的分泌。然而，目前还不清楚不同的研究人员，如果他们的合成保持不变，或可以根据不同的代谢条件进行修改。胰腺蛋白质的分泌和合成被平行研究，以评估分泌是否触发合成，或者这两种现象是由不同的机制控制的。这些研究的方法主要包括体内和体外实验的结合。在体内注射刺激物，并收集不同时间段的胰腺分泌物。然后处死动物，并在体外沿着测量蛋白质合成以及分泌到孵育培养基中的淀粉酶。结果表明，在单次注射或输注胆囊收缩素-胰酶素（CCK-PZ）和促胰液素后的前15分钟内，蛋白质合成减少。 这一减少与一个...
The secretion of the various pancreatic enzymes can be increased by hormonal and cholinergic stimulation. However, it is not yet clear among the different investigators if their synthesis remains constant or can be modified according to different metabolic conditions. The secretion and synthesis of the pancreatic proteins were then studied in parallel to evaluate if secretion triggers synthesis or both phenomenons are controlled by separate mechanisms.The approach for these studies consists mainly in a combination of in vivo and in vitro experiments. The stimulants were injected in vivo and the pancreatic secretions were collected for different periods of time. The animals were then sacrificed and protein synthesis was measured in vitro along with the amylase secreted into the incubation medium. The results show that protein synthesis is decreased during the first 15 min after a single injection or infusion of both cholecystokinin–pancreozymin (CCK–PZ) and secretin. This reduction was associated with an i...