SPECIFIC HUMAN CYTOTOXIC T-CELLS RECOGNIZE B-CELL LINES PERSISTENTLY INFECTED WITH RESPIRATORY SYNCYTIAL VIRUS
SPECIFIC HUMAN CYTOTOXIC T-CELLS RECOGNIZE B-CELL LINES PERSISTENTLY INFECTED WITH RESPIRATORY SYNCYTIAL VIRUS
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DOI：
10.1073/pnas.83.23.9183
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
MCMICHAEL, AJ
中科院分区：
文献类型：
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作者：
BANGHAM, CRM;MCMICHAEL, AJ
T 淋巴细胞对呼吸道合胞 (RS) 病毒的反应已被用来解释 RS 病毒在人类婴儿中引起的细支气管炎和肺炎。然而，T 细胞似乎也在抵抗 RS 病毒感染方面发挥着作用。尽管RS病毒特异性人类淋巴细胞已被证实，但淋巴细胞的表型和功能均未得到表征。我们在此描述了在来自人外周血的大量培养物和再刺激细胞系中诱导抗RS病毒细胞毒性T淋巴细胞。 RS病毒在体外感染Epstein-Barr病毒转化的人类B细胞系很容易引起持续感染；这些细胞在感染后4个月继续合成RS病毒蛋白并分泌传染性RS病毒。持续感染的细胞既可用于重新刺激细胞毒性T细胞前体，又可作为RS病毒特异性细胞毒性T细胞的靶标。
The T-lymphocyte response to respiratory syncytial (RS) virus has been invoked to explain the bronchiolitis and pneumonia caused by RS virus in human infants. However, T cells also appear to play a role in protection against RS virus infection. Although RS virus-specific human lymphocytes have been demonstrated, neither the phenotype nor the function of the lymphocytes was characterized. We describe here the induction of anti-RS virus cytotoxic T lymphocyte, in both bulk culture and restimulated cell lines, from human peripheral blood. Infection of Epstein-Barr virus-transformed human B-cell lines with RS virus in vitro readily caused a persistent infection; these cells continued to synthesize RS viral proteins and secrete infectious RS virus 4 months after infection. The persistently infected cells were used both to restimulate cytotoxic-T-cell precursors and as targets for RS virus-specific cytotoxic T cells.