Prediction of oocyte quality using mRNA transcripts screened by RNA sequencing of human granulosa cells
Prediction of oocyte quality using mRNA transcripts screened by RNA sequencing of human granulosa cells
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使用通过人颗粒细胞 RNA 测序筛选的 mRNA 转录本预测卵母细胞质量
DOI：
10.1016/j.rbmo.2021.05.018
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发表时间：
2021-09-20
影响因子：
4
通讯作者：
Zhou, Xu
中科院分区：
文献类型：
--
作者：
Liu, Chengjun;Su, Ketong;Zhou, Xu
Research question: Can RNA transcripts of granulosa cells be used to assess oocyte quality? The possibility of predicting the developmental competence of oocytes by RNA sequencing analysis of granulosa cells was evaluated.Design: Granulosa cell samples were collected from 29 women undergoing assisted reproductive technology treatment and divided into two groups: 14 samples from the high blastocyst rate group and 15 from the low blastocyst rate group. Ten samples from each group were selected for RNA sequencing.Results: A total of 129 differentially expressed genes associated with high developmental competence of oocytes were identified. COL1A2, renin and COL1A1 were selected and further examined by quantitative real-time polymerase chain reaction (qRT-PCR). Expression levels of COL1A2 and renin by qRT-PCR were consistent with the results of RNA sequencing.Conclusion: RNA sequencing data could provide novel marker genes for the non-invasive evaluation of oocyte quality and embryo developmental competence.
