Interactions between stem cell factor and c-Kit are required for intestinal immune system homeostasis.
Interactions between stem cell factor and c-Kit are required for intestinal immune system homeostasis.
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DOI：
10.1016/s1074-7613(94)80015-4
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发表时间：
1994-12
期刊：
影响因子：
32.4
通讯作者：
L. Puddington;S. Olson;L. Lefrançois
中科院分区：
文献类型：
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作者：
L. Puddington;S. Olson;L. Lefrançois
干细胞因子（SCF）及其受体c-Kit之间的相互作用对于造血细胞、黑素细胞和生殖细胞的发育是重要的。在c-Kit（W/Wv）或SCF（SI/SId）突变小鼠中，T淋巴细胞显示正常，除了那些位于肠上皮内的上皮内淋巴细胞（IEL）。正常情况下，IEL由相同数量的具有αβ或γδ T细胞受体的细胞组成。在突变小鼠中，从6-8周龄开始，γδ IEL的数量减少，而αβ IEL增加。后者主要是由于CD 4 + CD 8 +TCRαβ亚群增加，表明这些细胞可能是αβ IEL谱系的中间体。c-Kit或SCF分别由IEL或肠上皮细胞表达，表明直接细胞间相互作用的潜力。这种可能性得到了证明c-Kit突变直接影响IEL的重建研究的支持。因此，SCF-c-Kit相互作用对于肠免疫区室的稳态是重要的。
Interactions between stem cell factor (SCF) and its receptor, c-Kit, are important for development of hematopoietic, melanocytes, and germ cells. T lymphocytes appeared normal in c-Kit (W/Wv) or SCF (SI/SId) mutant mice, except for those residing within the intestinal epithelium, the intraepithelial lymphocytes (IEL). Normally, IEL are composed of equal numbers of cells with αβ or γδ T cell receptors. In mutant mice, beginning at 6–8 weeks of age, the number of γδ IEL decreased, whereas αβ IEL increased. The latter was due largely to an increased CD4+CD8+TCRαβ subset, suggesting that these cells may be intermediates in the αβ IEL lineage. c-Kit or SCF was expressed by IEL or intestinal epithelial cells, respectively, indicating a potential for direct intercellular interaction. This possibility was supported by reconstitution studies that demonstrated that c-Kit mutations directly affected IEL. Thus, SCF—c-Kit interactions are important for homeostasis of the intestinal immune compartment.