Immature ovaries and polycystic kidneys in the congenital polycystic kidney mouse may be due to abnormal sex steroid metabolism.
Immature ovaries and polycystic kidneys in the congenital polycystic kidney mouse may be due to abnormal sex steroid metabolism.
复制标题
先天性多囊肾小鼠的卵巢未成熟和多囊肾可能是由于性类固醇代谢异常所致。
DOI：
10.1016/s0303-7207(00)00398-1
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发表时间：
2001
影响因子：
4.1
通讯作者：
Aziz,N
中科院分区：
文献类型：
--
作者：
Woo,D;Lee,GY;Anderson,E;Aziz,N
Ke 6是一种17β-羟基类固醇脱氢酶（17βHSD），在肾脏和性腺中表达。该基因的表达在隐性多囊肾病（一种发育障碍）的三种小鼠模型中显着降低，其中受影响肾脏内的一些肾单位发展成巨大的充满液体的囊肿，而非囊性肾单位通过细胞凋亡萎缩。在这里，我们发现在具有多囊肾的cpk/cpk小鼠中，雌性生殖器官也不能正常成熟，并在发育的早期阶段保持停滞。直接测量17βHSD活性显示cpk/cpk小鼠性腺和非性腺组织内雌激素和雄激素代谢严重减少。利用免疫荧光法定位了Ke 6蛋白在雌鼠生殖器官中的表达。我们的研究结果表明，雌激素/雄激素代谢可能在泌尿生殖系统的发育中起重要作用。
Ke 6 is a 17β-hydroxysteroid dehydrogenase (17βHSD) that is expressed in the kidneys and gonads. The expression of this gene is markedly reduced in three murine models of recessive polycystic kidney disease, a developmental disorder, where some nephrons within the affected kidneys develop into huge fluid-filled cysts while the non-cystic nephrons atrophies by apoptosis. Here, we show that in the cpk/cpk mouse, which have polycystic kidneys, the female reproductive organs also fail to mature properly and remain arrested at an early stage of development. Direct measurement of 17βHSD activity showed a severe reduction in estrogen and androgen metabolism within gonadal and non-gonadal tissues of the cpk/cpk mouse. Using immunofluorescent staining we localized the expression of the Ke 6 protein within the female mouse reproductive organs. Our findings suggest that estrogen/androgen metabolism may play an important role in the development of the urogenital systems.