Comparing PLFA and amino sugars for microbial analysis in an Upper Michigan old growth forest
Comparing PLFA and amino sugars for microbial analysis in an Upper Michigan old growth forest
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DOI：
10.1016/j.soilbio.2008.01.022
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发表时间：
2008-08
影响因子：
9.7
通讯作者：
C. Liang;R. Fujinuma;T. Balser
中科院分区：
文献类型：
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作者：
C. Liang;R. Fujinuma;T. Balser
比较了磷脂脂肪酸（PLFA）分析和氨基糖分析可靠指示微生物生物量和总体群落结构（例如真菌与细菌比率）的能力。我们对古老森林中主要树冠树种下方的三组土壤进行了采样，并使用 PLFA 和氨基糖程序进行提取。氨基糖数据在样品重复之间具有较低的变异系数，因此与 PLFA 数据相比可能更适合定量应用。然而，在考虑微生物群落结构时，PLFA 由于其更直接的解释而比氨基糖具有优势。最后，由于每种生物标志物源自微生物生物质的不同部分，因此细胞膜 PLFA 和细胞壁氨基糖之间的转换因子可能对理解土壤碳循环有价值。
Phospholipid fatty acid (PLFA) analysis and amino sugars analysis were compared with respect to their capacity to reliably indicate microbial biomass and gross community structure (e.g. fungi to bacteria ratio). We sampled three sets of soils beneath dominant canopy-tree species in an old growth forest and extracted them using both PLFA and amino sugars procedures. Amino sugars data had a lower coefficient of variation among sample replicates and therefore may be better for quantitative application compared to PLFA data. However, when considering microbial community structure, PLFA had an advantage over amino sugars due to its more straightforward interpretation. Finally, because each biomarker derives from different portions of the microbial biomass, a conversion factor between cell membrane PLFA and cell wall amino sugars may be valuable in understanding soil carbon cycling.