CONTROL OF ETHYLENE SYNTHESIS BY EXPRESSION OF A BACTERIAL ENZYME IN TRANSGENIC TOMATO PLANTS
CONTROL OF ETHYLENE SYNTHESIS BY EXPRESSION OF A BACTERIAL ENZYME IN TRANSGENIC TOMATO PLANTS
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DOI：
10.1105/tpc.3.11.1187
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发表时间：
1991-11-01
期刊：
影响因子：
11.6
通讯作者：
KISHORE, GM
中科院分区：
文献类型：
--
作者：
KLEE, HJ;HAYFORD, MB;KISHORE, GM
植物激素乙烯的合成被认为是许多植物发育过程所必需的。 跃变型水果和蔬菜的成熟控制是这些过程中最具特征的。 减少植物中乙烯合成的一种方法是其直接前体1-氨基环丙烷-1-羧酸（ACC）的代谢。 土壤细菌含有一种酶，ACC脱氨酶，通过它们在ACC上生长的能力作为唯一的氮源来鉴定。 将编码ACC脱氨酶的基因克隆并导入番茄植株中。 在转基因植物中乙烯合成的减少没有引起任何明显的营养表型异常。 然而，来自这些植物的果实表现出明显的成熟延迟，并且成熟果实比非转基因对照果实保持坚硬至少6周。 这些结果表明，ACC脱氨酶是有用的研究乙烯在许多发育和压力相关的过程中的植物，以及延长货架寿命的水果和蔬菜的成熟是由乙烯介导的作用。
Synthesis of the phytohormone ethylene is believed to be essential for many plant developmental processes. The control of ripening in climacteric fruits and vegetables is among the best characterized of these processes. One approach to reduce ethylene synthesis in plants is metabolism of its immediate precursor, 1-aminocyclopropane-1-carboxylic acid (ACC). Soil bacteria containing an enzyme, ACC deaminase, were identified by their ability to grow on ACC as a sole nitrogen source. The gene encoding ACC deaminase was cloned and introduced into tomato plants. Reduction in ethylene synthesis in transgenic plants did not cause any apparent vegetative phenotypic abnormalities. However, fruits from these plants exhibited significant delays in ripening, and the mature fruits remained firm for at least 6 weeks longer than the nontransgenic control fruit. These results indicated that ACC deaminase is useful for examining the role of ethylene in many developmental and stress-related processes in plants as well as for extending the shelf life of fruits and vegetables whose ripening is mediated by ethylene.