EVIDENCE FOR DIRECT ACTIONS OF GENERAL-ANESTHETICS ON AN ION-CHANNEL PROTEIN - A NEW LOOK AT A UNIFIED MECHANISM OF ACTION
EVIDENCE FOR DIRECT ACTIONS OF GENERAL-ANESTHETICS ON AN ION-CHANNEL PROTEIN - A NEW LOOK AT A UNIFIED MECHANISM OF ACTION
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DOI：
10.1097/00000542-199408000-00022
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发表时间：
1994-08-01
期刊：
影响因子：
8.8
通讯作者：
LIU, Y
中科院分区：
文献类型：
--
作者：
DILGER, JP;VIDAL, AM;LIU, Y
背景：离子通过烟碱乙酰胆碱受体通道的渗透被全身麻醉药抑制。这种抑制作用可以通过麻醉剂分子与通道蛋白本身的结合或麻醉剂对蛋白脂质环境的影响来介导。方法：采用膜片钳技术研究乙醚和丙泊酚对BC 3 H-1细胞外向型膜片上乙酰胆碱受体通道的影响。测量了单通道的动力学和电导性质。一个快速的灌注系统被用来在膜片钳记录过程中，以确定由麻醉剂的抑制动力学的麻醉剂浓度的快速变化。结果：乙醚，异氟烷（从以前的研究结果），异丙酚产生不同的动力学模式的单一乙酰胆碱受体通道的活动。乙醚降低通道的表观电流振幅，异氟烷诱导闪烁通道活动，异丙酚仅减少通道的开放时间。这些麻醉剂的抑制动力学也不同。以太（
Background: Ion permeation through the nicotinic acetylcholine receptor channel is inhibited by general anesthetics. This inhibition could be mediated either by binding of anesthetic molecules to the channel protein itself or by the effects of anesthetics on the lipid environment of the protein.Methods: Patch clamp recording techniques were used to investigate the effects of ether and propofol on acetylcholine receptor channels in outside-out patches from BC3H-1 cells. The kinetic and conductance properties of single channels were measured. A rapid perfusion system was used to make rapid changes in anesthetic concentration during patch clamp recording to determine the kinetics of inhibition by anesthetics.Results: Ether, isoflurane (results from previous studies), and propofol produce distinct kinetic patterns of single acetylcholine receptor channel activity. Ether reduces the apparent current amplitude of channels, isoflurane induces flickering channel activity and propofol merely decreases the open time of the channel. The kinetics of inhibition are also different for these anesthetics. Ether (