RNA COMPLEMENTARY TO A HERPESVIRUS ALPHA-GENE MESSENGER-RNA IS PROMINENT IN LATENTLY INFECTED NEURONS
RNA COMPLEMENTARY TO A HERPESVIRUS ALPHA-GENE MESSENGER-RNA IS PROMINENT IN LATENTLY INFECTED NEURONS
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DOI：
10.1126/science.2434993
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发表时间：
1987-02-27
期刊：
影响因子：
56.9
通讯作者：
FELDMAN, LT
中科院分区：
文献类型：
--
作者：
STEVENS, JG;WAGNER, EK;FELDMAN, LT
在最初试图确定导致单纯疱疹病毒潜伏状态的分子事件时，利用原位杂交在潜伏感染的感觉神经元中寻找病毒编码的RNA转录本。使用代表整个病毒基因组的克隆探针表明，存在编码在末端重复序列中的转录物。当.阿尔法。使用编码ICP0、ICP4和ICP27的基因以及编码VP-5的γ1基因，仅检测到与ICP0探针杂交的RNA转录本。在潜伏感染的细胞中，与ICP-0相关的转录本主要定位在细胞核中；这在急性(有效)感染的神经元或被探测到编码肌动蛋白的RNA转录本的神经元中并非如此。在Northern印迹实验中，用ICP0探针检测到2.6kb的RNA。当以来自ICP0区的单链DNA为探针时，与编码ICP0信使RNA(MRNA)互补的单链RNA是检测到的主要物种。这种RNA物种可能在维持潜伏感染方面发挥重要作用。
In initial attempts to define the molecular events responsible for the latent state of herpes simplex virus, in situ hybridization was utilized to search for virally encoded RNA transcripts in latently infected sensory neurons. The use of cloned probes representing the entire viral genome indicated that transcrips encoded within terminal repeat were present. When the .alpha. genes encoding ICP-0, ICP-4, and ICP-27 and the .gamma.1 gene encoding VP-5 were employed, only RNA transcripts hybridizing to the ICP-0 probe were detected. In latently infected cells, the ICP-0-related transcripts were localized principally in the nucleus; this was not the case in acutely (productively) infected neurons or in neurons probed for RNA transcripts coding for actin. In Northern blotting experiments, an RNA of 2.6 kilobases was detected with the ICP-0 probe. When single-stranded DNAs from the ICP-0 region were used as probes, RNA from the strand complementary to that encoding ICP-0 messenger RNA (mRNA) was the major species detected. This RNA species may play a significant role in maintaining the latent infection.