Copper-induced hydrogen peroxide upregulation of a metallothionein gene, OsMT2c, from Oryza sativa L. confers copper tolerance in Arabidopsis thaliana
Copper-induced hydrogen peroxide upregulation of a metallothionein gene, OsMT2c, from Oryza sativa L. confers copper tolerance in Arabidopsis thaliana
复制标题
铜诱导的过氧化氢上调来自稻的金属硫蛋白基因 OsMT2c，赋予拟南芥铜耐受性
DOI：
10.1016/j.jhazmat.2015.03.060
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发表时间：
2015-08-30
影响因子：
13.6
通讯作者：
Shen, Zhenguo
中科院分区：
文献类型：
--
作者：
Liu, Jia;Shi, Xiaoting;Shen, Zhenguo
金属硫蛋白（MT）是一种低分子量、富含半胱氨酸的金属结合蛋白，广泛存在于许多属和种中，但其在非生物胁迫耐受中的功能尚不清楚。在这里，MT基因从水稻，OsMT 2c，分离和表征，编码2型MT，并观察到表达的根，叶鞘，叶，但只有在种子中弱表达。铜（Cu）和过氧化氢（H2 O2）处理使OsMT 2c表达上调。过量Cu诱导水稻体内H2 O2的产生和释放，外源H2 O2清除剂N，N '-二甲基硫脲（DMTU）和抗坏血酸（Asc）抑制水稻体内H2 O2的产生和OsMT 2c的表达。此外，OsMT 2c的表达在osapx 2突变体中增加，其中H2 O2水平高于野生型（WT）植物。这些结果表明，铜通过铜诱导的H2 O2在O. sativa.此外，与WT和空载体（Ev）幼苗相比，转OsMT 2c基因过表达的拟南芥表现出对Cu胁迫的耐受性提高，并且表现出活性氧（ROS）清除能力增加。(C)2015 Elsevier B. V.版权所有。
Metallothioneins (MTs) are low-molecular-weight, cysteine-rich metal-binding proteins found in numerous genera and species, but their functions in abiotic stress tolerance remain unclear. Here, a MT gene from Oryza sativa, OsMT2c, was isolated and characterized, encoding a type 2 MT, and observed expression in the roots, leaf sheathes, and leaves, but only weak expression in seeds. OsMT2c was upregulated by copper (Cu) and hydrogen peroxide (H2O2) treatments. Excessive Cu elicited a rapid and sustained production and release of H2O2 in rice, and exogenous H2O2 scavengers N,N'-dimethylthiourea (DMTU) and ascorbic acid (Asc) decreased H2O2 production and OsMT2c expression. Furthermore, the expression of OsMT2c increased in the osapx2 mutant in which the H2O2 levels were higher than in wild-type (WT) plants. These results showed that Cu increased MT2c expression through the production and accumulation of Cu-induced H2O2 in O. sativa. In addition, the transgenic OsMT2c-overexpressing Arabidopsis displayed improved-tolerance to Cu stress and exhibited increased reactive oxygen species (ROS) scavenging ability compared to WT and empty-vector (Ev) seedlings. (C) 2015 Elsevier B.V. All rights reserved.