The Features of Inflammation Factors Concentrations in Aqueous Humor of Polypoidal Choroidal Vasculopathy.
The Features of Inflammation Factors Concentrations in Aqueous Humor of Polypoidal Choroidal Vasculopathy.
复制标题
息肉样脉络膜血管病房水炎症因子浓度特征
DOI：
10.1371/journal.pone.0147346
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Lu L
中科院分区：
文献类型：
--
作者：
Hu J;Leng X;Hu Y;Atik A;Song X;Li Z;Liu Y;Lu L
目的探讨难治性息肉样脉络膜血管病(PCV)患者房水中细胞因子的浓度变化。研究对象分为三组：难治性PCV(A组，41只眼)、稳定PCV(B组，39只眼)和老年性白内障(C组，44只眼)。A组和B组在两个时间点采集房水样本--第一次玻璃体内注射雷尼比单抗之前和最后一次注射之前。C组于超声乳化术前取房水标本，用流式细胞仪和流式细胞仪检测细胞因子IL-2、IL-6、IL-8、肿瘤坏死因子α、单核细胞趋化蛋白1、血管内皮生长因子α的浓度。A组治疗前单核细胞趋化蛋白-1、血管内皮生长因子、肿瘤坏死因子-α水平显著高于C组(P<0.05)，且A组显著高于B组(P<0.05)。B组MCP-1和VEGF水平明显高于C组(P<；0.05)。A组治疗前血清单核细胞趋化蛋白-1、血管内皮生长因子和肿瘤坏死因子-α水平显著高于B组和C组(P<0.05)。A组IL-2水平显著低于B组(P<；0.05)和C组(P<；0.05)。炎性细胞因子如单核细胞趋化蛋白-1、血管内皮生长因子和肿瘤坏死因子-α可能与稳定和难治性PCV的发病有关。
To investigate the cytokine concentrations in the aqueous humor of patients with refractory polypoidal choroidal vasculopathy (PCV). Three separate groups of patients were studied–refractory PCV (Group A, 41 eyes), stable PCV (Group B, 39 eyes) and senile cataract (Group C, 44 eyes). Aqueous humor samples were collected at two time points for Groups A and B–before the first intravitreal ranibizumab injection and before the last injection. Aqueous humor samples were collected prior to phacoemulsification in Group C. The cytokine concentrations of interleukin 2, 6, and 8 (IL-2, IL-6, and IL-8), tumor necrosis factor α (TNF-α), monocyte chemotactic protein 1 (MCP-1), and vascular endothelial growth factor (VEGF) were measured by cytometric bead array and flow cytometry. Before the first treatment, the MCP-1, VEGF, and TNF-α levels in Group A were significantly higher than those in Group C (P < 0.05), and the MCP-1 and VEGF levels in Group A were significantly higher than those in Group B (P < 0.05). Significantly higher MCP-1 and VEGF levels were seen in Group B compared to Group C (P < 0.05). Before the final treatment, the MCP-1, VEGF, and TNF-α concentrations in Group A were significantly higher than those in Group B (P < 0.05) and Group C (P < 0.05). IL-2 levels were significantly lower in Group A compared to Group B (P < 0.05) and Group C (P < 0.05). Inflammatory cytokines such as MCP-1, VEGF, and TNF-α may be associated with the pathogenesis of both stable and refractory PCV.