SERIAL CULTIVATION OF HUMAN DIPLOID CELL STRAINS
SERIAL CULTIVATION OF HUMAN DIPLOID CELL STRAINS
复制标题
DOI：
10.1016/0014-4827(61)90192-6
复制
发表时间：
1961-01-01
影响因子：
3.7
通讯作者：
MOORHEAD, PS
中科院分区：
文献类型：
--
作者：
HAYFLICK, L;MOORHEAD, PS
本文报道了25株人胎儿二倍体成纤维细胞的分离和鉴定。采用常规组织培养技术。除了维持二倍体核型外，还有10个其他标准用于区分这些菌株与异倍体细胞系。这些包括性染色质的保留、组织型分化、对悬浮培养的不适应性、体内非恶性特征、培养的有限限制、与原代组织相似的病毒谱、与细胞系相比增加的产酸、科萨基A9受体物质的保留以及菌株易于开发。细胞株在70 ℃下存活并保留所有特征，确保了几乎无限的任何菌株供应，而不管它们在约50次传代和培养1年后退化的事实。考虑到这些菌株最终退化的原因，消除了诸如培养基组分、潜伏病毒和PPLO以及一些非复制细胞内组分的消耗等因素。得出的结论是，这种现象是由于内在的细胞因素，这是表示为在细胞水平上的衰老。由于这些特性及其极广的病毒谱，建议使用二倍体人细胞株生产人病毒疫苗。鉴于这些观察结果，细胞培养学家使用的一些术语被重新定义。
The isolation and characterization of 25 strains of human diploid fibroblasts derived from fetuses are described. Routine tissue culture technics were employed. Other than maintenance of the diploid karyotype, 10 other criteria serve to distinguish these strains from heteroploid cell lines. These include retention of sex chromatin, histotypical differentiation, non-adaptibility to suspended culture, non-malignant characteristics in vivo, finite limit of cultivation, similar virus spectrum to primary tissue, increased acid production compared to cell lines, retention of Coxsackie A9 receptor substance, and ease with which strains can be developed. Survival of cell strains at[long dash]70C with retention of all characteristics insures an almost unlimited supply of any strain regardless of the fact that they degenerate after about 50 subcultivations and 1 year in culture. A consideration of the cause of the eventual degeneration of these strains eliminates such factors as media components, latent viruses and PPLO, and depletion of some non-replicating intracellular component. The conclusion reached is that the phenomenon is attributable to intrinsic cellular factors which are expressed as senescence at the cellular level. With these characteristics and their extremely broad virus spectrum, the use of diploid human cell strains for human virus vaccine production is suggested. In view of these observations a number of terms used by cell culturists are redefined.