Xylene-Free Method for Histological Preparation: A Multicentre Evaluation
Xylene-Free Method for Histological Preparation: A Multicentre Evaluation
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DOI：
10.1038/labinvest.3780335
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发表时间：
2001-09
影响因子：
5
通讯作者：
Lars Falkeholm;C. A. Grant;A. Magnusson;Eva Möller
中科院分区：
文献类型：
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作者：
Lars Falkeholm;C. A. Grant;A. Magnusson;Eva Möller
日常病理诊断工作的支柱是石蜡切片。 150 多年来，制备石蜡切片的方法基本上没有改变。已经开发出一种无二甲苯方法，该方法不使用二甲苯，不仅作为石蜡浴之前的中间步骤，而且还用于切割切片的脱蜡，这也消除了染色和安装步骤中的再水化和脱水的需要。组织加工过程中消除二甲苯可降低成本、节省时间并改善实验室环境。 Vrinnevi 医院自 1995 年以来在无二甲苯科室方面的经验非常丰富。我们的观点是，不含二甲苯的切片与传统处理的切片相当。为了验证这一假设，来自三家医院的九名病理学家参加了一项评估试验。来自 10 个连续提交的乳房、肠道和皮肤样本的配对组织块通过无二甲苯或传统方法进行处理。将每个块的切片脱蜡并用苏木精-伊红 (H&E)、高碘酸-希夫 (PAS) 和 van Gieson 方法染色。 180 个切片（10 个样本× 3 个组织× 3 个染色剂× 2）的随机混合得到 90 个匹配对。每个切片均由 9 名病理学家进行盲检和评分，给出 810 个配对观察结果进行统计评估。在 74% 的比较中，无二甲苯切片与传统切片一样好或更好，26% 的比较较差。主要的区别因素是染色方法。 H&E 和 PAS 部分是等效的。从相同块上切下并随机分配给该染色剂的无二甲苯范吉森切片往往会被降级。这可以追溯到该批次使用的错误染色溶液。总体结果表明，无二甲苯处理组织切片的方法得到了专业人士的认可。
The backbone of daily pathological diagnostic work is the paraffin section. Paraffin sections are still prepared by methods largely unchanged for over 150 years. A xylene-free method has been developed that excludes xylene, not only as the intermediate step before the paraffin baths, but also for deparaffinizing of the cut sections, which also eliminates the need for rehydration and dehydration for the staining and mounting steps. Elimination of xylene from tissue processing cuts costs, saves time, and improves the laboratory environment. Experience with xylene-free sections since 1995 at the Vrinnevi Hospital is favorable. Our opinion is that the xylene-free sections are equivalent to conventionally processed sections. To test this hypothesis, nine pathologists from three hospitals participated in an evaluation trial. Paired tissue blocks from 10 consecutively submitted samples each of breast, intestine, and skin were processed by either the xylene-free or the conventional method. Sections from each block were deparaffinized and stained with hematoxylin-eosin (H&E), with periodic acid-Schiff (PAS), and with van Gieson’s method. A randomized mix of 180 sections (10 samples× 3 tissues× 3 stains× 2) gave 90 matched pairs. Each section was blindly examined and scored by nine pathologists to give 810 paired observations for statistical evaluation. The xylene-free sections were ranked as good as or better than their conventional counterparts in 74% of the comparisons, and poorer in 26%. The major discriminating factor was the staining method. H&E and PAS sections were equivalent. The xylene-free van Gieson sections, cut from the same blocks and randomly assigned to this stain, tended to be downgraded. This could be traced to a faulty stain solution used for this batch. The overall results have demonstrated professional acceptance for the xylene-free method of processing histological sections.