Tissue distribution of hepatopoietin-A: a heparin-binding polypeptide growth factor for hepatocytes.
Tissue distribution of hepatopoietin-A: a heparin-binding polypeptide growth factor for hepatocytes.
复制标题
肝生成素-A 的组织分布：肝素结合的肝细胞多肽生长因子。
DOI：
10.1073/pnas.87.3.1252
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发表时间：
1990
影响因子：
11.1
通讯作者：
Michalopoulos,G
中科院分区：
文献类型：
--
作者：
Zarnegar,R;Muga,S;Rahija,R;Michalopoulos,G
肝细胞生成素-A（HPTA）是一种肝素结合多肽生长因子，由分子量分别为70，000和35，000的重链和轻链多肽组成。在无血清培养基中刺激正常大鼠肝细胞原代培养物的DNA合成。从兔血清中完全纯化和鉴定HPTA，我们在别处报道过。最近，我们已经确定了兔HPTA轻链的氨基末端氨基酸序列多达24个残基，并表明该序列与其他已知序列不同源。[注：人类肝细胞生长因子，最近由另外两个小组测序，与HPTA是相同的分子种类。在本文中，我们报告了生产的中和多克隆抗血清中提出的鸡抗纯化兔HPTA。该抗血清不抑制肝细胞DNA合成的其他强效诱导剂（表皮生长因子或酸性成纤维细胞生长因子）的促有丝分裂作用，也不与酶联免疫吸附试验（ELISA）中的这些生长因子相互作用。抗体识别HPTA，如通过Western免疫印迹所确定的。由于HPTA的组织来源尚不清楚，因此，我们用抗HPTA抗血清，通过荧光染色法研究了HPTA在家兔体内的组织分布。胰腺的腺泡细胞、脑的神经元、甲状腺的C细胞、唾液腺的导管细胞和十二指肠的Brunners腺用抗HPTA抗体染色。肝脏、脾脏、胸腺和肾脏似乎不含明显量的HPTA。我们通过从上述染色组织中提取和纯化活性HPTA证实了这些发现。抗HPTA抗体识别从不同组织纯化的HPTA，如通过ELISA、Western免疫印迹和免疫中和实验所确定的。
Hepatopoietin-A (HPTA) is a heparin-binding polypeptide growth factor which consists of a heavy and a light polypeptide chain with molecular weights of 70,000 and 35,000, respectively. It stimulates DNA synthesis in primary cultures of normal rat hepatocytes in serum-free medium. The complete purification and characterization of HPTA from rabbit serum were reported by us elsewhere. Recently we have determined the amino-terminal amino acid sequence of the rabbit HPTA light chain up to 24 residues and have shown that the sequence is not homologous with other known sequences. [N.B. Human hepatocyte growth factor, recently sequenced by two other groups, is the same molecular species as HPTA.] In the present paper we report the production of a neutralizing polyclonal antiserum raised in chicken against purified rabbit HPTA. This antiserum does not inhibit the mitogenic effect of other potent inducers of hepatocyte DNA synthesis (epidermal growth factor or acidic fibroblast growth factor), nor does it interact with these growth factors in an enzyme-linked immunosorbent assay (ELISA). The antibody recognizes HPTA, as was determined by Western immunoblotting. Since the tissue origin of HPTA is not known, this anti-HPTA antiserum was used to investigate the tissue distribution of HPTA in rabbits by immunohistostaining methods. Acinar cells of the pancreas, neurons of the brain, C cells of the thyroid, ductal cells of the salivary glands, and Brunners glands of the duodenum stained with anti-HPTA antibody. Liver, spleen, thymus, and kidney do not seem to contain appreciable amounts of HPTA. We confirmed these findings by extracting and purifying active HPTA from the stained tissues listed above. The anti-HPTA antibody recognizes HPTA purified from different tissues, as was determined by ELISA, Western immunoblotting, and immunoneutralization experiments.