PROTOPINE INHIBITS HETEROTYPIC CELL ADHESION IN MDA-MB-231 CELLS THROUGH DOWN-REGULATION OF MULTI-ADHESIVE FACTORS
PROTOPINE INHIBITS HETEROTYPIC CELL ADHESION IN MDA-MB-231 CELLS THROUGH DOWN-REGULATION OF MULTI-ADHESIVE FACTORS
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DOI：
10.4314/ajtcam.v11i2.28
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Gao, Jian-Li
中科院分区：
文献类型：
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作者：
He, Kai;Gao, Jian-Li
背景：延胡索是一种中草药。Wang在我们前期的研究中显示了抗肿瘤和抗血管生成的作用。材料与方法：采用MTT法、细胞迁移实验、细胞侵袭实验和三种细胞粘附实验，研究延胡索生物碱对人乳腺癌细胞MDA-MB-231的体外抗肿瘤作用。通过Western印迹法检测表皮生长因子受体（EGFR）、细胞间粘附分子1（ICAM-1）、α v-整合素、β（1）-整合素和β（5）-整合素的表达，进一步探讨化合物抑制异型细胞粘附的机制。在5种供试生物碱中，只有原阿片碱对MDA-MB-231细胞具有抗粘附和抗侵袭作用，这可能是延胡索抗肿瘤转移作用的重要机制之一。结果表明，Protopine作用90 min后，EGFR、ICAM-1、α v-整合素、β 1-整合素和β 5-整合素的表达均明显降低。结论：Protopine可能通过改变粘附因子的表达，抑制MDA-MB-231细胞与人脐静脉内皮细胞的异型粘附。
Background: A Chinese herb Corydalis yanhusuo W.T. Wang that showed anticancer and anti-angiogenesis effects in our previous studies was presented for further studies. In the present study, we studied the anticancer proliferation and adhesion effects of five alkaloids which were isolated from Corydalis yanhusuo.Materials and Methods: MTT dose response curves, cell migration assay, cell invasion assay, as well as three types of cell adhesive assay were performed on MDA-MB-231 human breast cancer cells. The mechanism of the compounds on inhibiting heterotypic cell adhesion were further explored by determining the expression of epidermal growth factor receptor (EGFR), Intercellular adhesion molecule 1 (ICAM-1), alpha v-integrin, beta(1)- integrin and beta(5)-integrin by western blotting assay.Results: In five tested alkaloids, only protopine exhibited anti-adhesive and anti-invasion effects in MDA-MB-231 cells, which contributed to the anti-metastasis effect of Corydalis yanhusuo. The results showed that after treatment with protopine for 90 min, the expression of EGFR, ICAM-1, av-integrin, beta(1)- integrin and beta(5)- integrin were remarkably reduced.Conclusion: The present results suggest that protopine seems to inhibit the heterotypic cell adhesion between MDA-MB-231 cells, and human umbilical vein endothelial cells by changing the expression of adhesive factors.