S-RNases from Self-incompatible and -compatible Apple Cultivars: Purification, Cloning, Enzymic Properties, and Pollen Tube Growth Inhibitory Activity-猫眼课题宝

DOI：

10.1271/bbb.66.1185

发表时间：

2002-01

期刊：

Bioscience, Biotechnology, and Biochemistry

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Naoki Katoh;K. Goto;Junpei Asano;K. Fukushima;Kenji Yamada;A. Kasai;T. Li;Makoto Takanoha;K. Miyairi;T. Okuno

从两个苹果品种（Malus arctica）的花柱中分离纯化了4种与自交不亲和性相关的核糖核酸酶（S-RNase），Starking Delicious（SD）和一个自交的CV，Megumi（MG）.每个品种产生两个S-RNases和他们的酶的特性，如比活性，pH值最佳，热稳定性，和分子量，进行了表征。在离体生物测定中，4种S-RNases在25 μg/ml（1.0 μM）浓度下抑制苹果花粉的花粉管生长，但不能区分自花花粉和非自花花粉。克隆了4个S-RNase的cDNA，并对其核苷酸序列和推导的氨基酸序列进行了分析。SD-Se RNase的核苷酸序列为一新序列，另一序列与cv.富士。在MG中，一个与SD-Sc RNase的序列相同，另一个与cv的Sa-RNase的序列相同。金冠除了一个基地。从花柱粗提物的分离量和Western blot分析结果来看，MG中S-RNases的含量明显低于SD。

Four S-RNases (RNase associated with self-incompatibility) were purified from the styles of two apple cultivars (Malus domestica), a self-incompatible cv., Starking Delicious (SD), and a self-compatible cv., Megumi (MG). Each cultivar produced two S-RNases and their enzymatic properties such as specific activity, pH optimum, thermal stability, and molecular mass, were characterized. The four S-RNases inhibited the tube growth of apple pollen in an in vitro bioassay at 25 μg/ml (1.0 μM), but did not distinguish self from non-self pollen. The cDNAs of four S-RNases were cloned, and the nucleotide and deduced amino acid sequences were analyzed. The nucleotide sequence of SD-Se RNase was a new one and the other was identical to that of Sc-RNase of cv. Fuji. In MG one was identical to the sequence of SD-Sc RNase and the other to that of Sa-RNase of cv. Golden Delicious except for one base. From results of the isolation amounts and the Western blot analysis for stylar crude extracts the amount of S-RNases in MG was apparently less than that in SD.

S-RNases from Self-incompatible and -compatible Apple Cultivars: Purification, Cloning, Enzymic Properties, and Pollen Tube Growth Inhibitory Activity

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