Analysis of Individual Mouse Activity in Group Housed Animals of Different Inbred Strains using a Novel Automated Home Cage Analysis System.
Analysis of Individual Mouse Activity in Group Housed Animals of Different Inbred Strains using a Novel Automated Home Cage Analysis System.
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DOI：
10.3389/fnbeh.2016.00106
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发表时间：
2016
影响因子：
3
通讯作者：
Armstrong JD
中科院分区：
文献类型：
--
作者：
Bains RS;Cater HL;Sillito RR;Chartsias A;Sneddon D;Concas D;Keskivali-Bond P;Lukins TC;Wells S;Acevedo Arozena A;Nolan PM;Armstrong JD
中枢神经系统疾病如自闭症以及神经退行性疾病如亨廷顿氏病的范围通常使用遗传改变的小鼠模型进行研究。目前用于表征这些小鼠的系统通常包括将动物从其饲养笼环境中移出，并将它们放入新的环境中，在那里它们接受一系列测试，测量一系列行为和身体表型。这些测试通常只在社会隔离的短时间内进行。然而，这类疾病的人类表现往往具有多种表型的特点，在很长一段时间内出现，并导致重大的社会影响。在这里，我们开发了一个系统，该系统将允许自动监测在已建立的社会群体内和长时间内饲养和圈养在笼子中的个体小鼠。我们证明，该系统准确地报告了群体内的个体运动行为，所采取的测量可以提供独特的见解，遗传背景对个体和群体行为的影响，以前没有认识到。
Central nervous system disorders such as autism as well as the range of neurodegenerative diseases such as Huntington's disease are commonly investigated using genetically altered mouse models. The current system for characterizing these mice usually involves removing the animals from their home-cage environment and placing them into novel environments where they undergo a battery of tests measuring a range of behavioral and physical phenotypes. These tests are often only conducted for short periods of times in social isolation. However, human manifestations of such disorders are often characterized by multiple phenotypes, presented over long periods of time and leading to significant social impacts. Here, we have developed a system which will allow the automated monitoring of individual mice housed socially in the cage they are reared and housed in, within established social groups and over long periods of time. We demonstrate that the system accurately reports individual locomotor behavior within the group and that the measurements taken can provide unique insights into the effects of genetic background on individual and group behavior not previously recognized.