Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver.
Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver.
复制标题
Cl-对短期培养大鼠肝细胞和离体灌注大鼠肝脏中有机阴离子转运的影响。
DOI：
10.1172/jci112946
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Sosiak,A
中科院分区：
文献类型：
--
作者：
Wolkoff,AW;Samuelson,AC;Johansen,KL;Nakata,R;Withers,DM;Sosiak,A
在短期培养的大鼠肝细胞中研究了35 S-标记的磺溴酞[35 S]BSP在牛血清白蛋白中的转运。在37 ℃时，[35 S]BSP的初始摄取是4 ℃时的5-10倍，至少15 min呈线性，可饱和，受胆红素抑制，在ATP耗尽或培养基中用蔗糖等渗取代NaCl后减少70%以上。用K+或Li+代替Na+并不改变吸收，而用HCO-3或葡萄糖酸盐代替Cl-使吸收减少约40%。用渗透性更强的NO-3取代Cl-可使BSP的初始吸收增加30%。ATP耗竭或蔗糖替代后，[35 S]BSP从细胞到培养基的流出被抑制40%。为了证实这些结果在一个更生理系统，运输[3 H]胆红素进行了研究，在离体肝脏灌注对照培养基或介质中，其中Cl-被取代葡萄糖酸盐-。通过Goresky模型分析的灌注数据显示，葡萄糖酸盐替代使流入和流出减少40-50%。这些结果是一致的存在的Cl-/有机阴离子交换机制类似于其他人所描述的肾小管。
Transport of 35S-labeled sulfobromophthalein [35S]BSP was studied in short-term cultured rat hepatocytes incubated in bovine serum albumin. At 37 degrees C, initial uptake of [35S]BSP was 5-10-fold that at 4 degrees C, linear for at least 15 min, saturable, inhibited by bilirubin, and reduced by greater than 70% after ATP depletion or isosmotic substitution of sucrose for NaCl in medium. Replacement of Na+ by K+ or Li+ did not alter uptake, whereas replacement of Cl- by HCO-3 or gluconate- reduced uptake by approximately 40%. Substitution of Cl- by the more permeant NO-3 enhanced initial BSP uptake by 30%. Efflux of [35S]BSP from cells to media was inhibited by 40% after ATP depletion or sucrose substitution. To confirm these results in a more physiologic system, transport of [3H]bilirubin was studied in isolated livers perfused with control medium or medium in which Cl- was replaced by gluconate-. Perfusion data analyzed by the model of Goresky, revealed 40-50% reductions in influx and efflux with gluconate- substitution. These results are consistent with existence of a Cl-/organic anion-exchange mechanism similar to that described by others in renal tubules.