Eradication of cross-contaminated cell lines: A call for action
Eradication of cross-contaminated cell lines: A call for action
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DOI：
10.1007/s10565-007-9019-9
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发表时间：
2007-11-01
影响因子：
6.1
通讯作者：
Nardone, Roland M.
中科院分区：
文献类型：
--
作者：
Nardone, Roland M.
这份“白皮书”是编写并广泛传播的，目的是对长期存在的一个严重的、悬而未决的、日益严重的问题敲响警钟，这个问题影响到生物医学研究的很大一部分，即使用被错误识别和交叉污染的细胞培养物。这份“白皮书”展示了大胆的行动如何在整个行业的实践中带来改变，从而防止进一步的侵蚀。研究中使用的哺乳动物细胞培养的错误识别以及种内和种内交叉污染仍然是一个普遍存在的问题，尽管人们对此的认识可以追溯到45年前。对这一问题的认识使人们对交叉污染的原因有了很好的了解，并采取了适当的预防措施。它还导致了用于细胞系鉴定的可靠方法的应用。然而，这个问题仍然有增无减。据估计，由于使用错误识别和交叉污染的细胞培养而出现缺陷的研究论文的发生率约为15%-20%。形势的严重性要求采取一种战略，向几乎所有细胞培养研究人员传递认证的补救信息，并确保遵守该信息。这里提出的战略的核心是将细胞系鉴定作为授予研究补助金和发表研究成果的条件。
This "white paper" was prepared and widely disseminated in an attempt to sound an alarm about the long-term existence of a grave, unresolved and growing problem that affects a significant portion of biomedical research, namely, the use of misidentified and cross-contaminated cell cultures. The "white paper" shows how bold action could bring about a profession-wide change in practice that would prevent further erosion. Misidentification and inter- and intra-specific cross-contamination of mammalian cell cultures used in research continues as a widespread problem despite an awareness that dates back more than 45 years. Awareness of the problem has led to a good understanding of the causes of cross-contamination and appropriate preventive measures. It has also led to the application of robust methods for the authentication of cell lines. Yet the problem continues unabated. Estimates of the incidence of research papers flawed by the use of misidentified and cross-contaminated cell cultures approximate 15-20%. The gravity of the situation calls for a strategy that would deliver a remedial message of authentication to virtually all cell culture researchers and also ensure compliance with the message. At the core of the strategy proposed herein is having cell line authentication as a condition for the award of research grants and for the publication of research findings.