Interactions between rickettsiae and Dermacentor variabilis ticks: analysis of gene expression.
Interactions between rickettsiae and Dermacentor variabilis ticks: analysis of gene expression.
复制标题
立克次体和变异革蜱之间的相互作用：基因表达分析。
DOI：
10.1111/j.1749-6632.2003.tb07428.x
复制
发表时间：
2003
影响因子：
5.2
通讯作者：
Azad,AbduF
中科院分区：
文献类型：
--
作者：
Macaluso,KevinR;Mulenga,Albert;Simser,JasonA;Azad,AbduF
摘要：蜱传斑疹热群立克次体在自然界中主要通过其媒介跨体和跨卵巢传播。在蜱虫中，卵巢的感染和持续存在是立克次体从一代到下一代传播周期的关键步骤。尽管蜱蜱可以通过实验维持多种立克次体，但通常只能通过卵巢维持单一物种。排除过程的分子机制尚不清楚。在这项研究中，尝试鉴定可能参与立克次体对蜱细胞粘附的分子。使用针对哺乳动物整联蛋白α和β亚基的单克隆抗体来筛选由未感染的蜱卵巢组织构建的λ噬菌体文库。鉴定出了α和β整合素的阳性斑块。对于一种整合素β3，分离噬菌斑并对插入片段进行测序。讨论了通过文库筛选鉴定的这些分子在立克次体感染和存活中的作用。
Abstract:Tick‐borne spotted fever group rickettsiae are maintained in nature primarily through transstadial and transovarial transmission in their vector. In the tick,Dermacentor variabilis, the infection of, and persistence within, the ovaries are critical steps in the transmission cycle of rickettsiae from one generation to the next. Although ixodid ticks can experimentally maintain several species of rickettsiae, often only a single species is maintained transovarially. The molecular mechanisms underlying exclusion processes are unknown. In this study, an attempt to identify the molecules that may be involved in rickettsial adhesion to tick cells was carried out. Monoclonal antibodies to mammalian alpha and beta subunits of integrins were used to screen a lambda phage library, constructed from uninfected tick ovarian tissues. Positive plaques for both alpha and beta integrins were identified. For one integrin, β3, the plaque was isolated and the insert sequenced. The role of these molecules, identified by library screening, in rickettsial infection and survival are discussed.