Genome Characteristics and Evolution of Pseudorabies Virus Strains in Eastern China from 2017 to 2019
Genome Characteristics and Evolution of Pseudorabies Virus Strains in Eastern China from 2017 to 2019
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DOI：
10.1007/s12250-019-00140-1
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发表时间：
2019-12-01
影响因子：
5.5
通讯作者：
Gu, Jinyan
中科院分区：
文献类型：
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作者：
Zhai, Xiaofeng;Zhao, Wen;Gu, Jinyan
自2011年底以来，中国南方爆发了伪狂犬病毒（PRV），给养猪业造成了重大经济损失。我们以前报道过，中国的PRV变体形式和重组可能是持续流行的来源。在此，我们分析了2017 - 2019年中国东部集约化养猪场的样本，并对主要糖蛋白（gB, gC， gD和gE）进行了测序，以研究PRV的进化特征。基于gC基因，我们发现PRV变异属于进化支2，并在PRV流行期间检测到奠基者效应。此外，我们还检测到了枝间和枝内的重组；特别是菌株FJ-ZXF和FJ-W2的gB基因在枝间重组，这两个菌株与枝1菌株进行了重组。我们还发现了特定的氨基酸变化和积极选择的位点，可能与功能变化有关。对中国出现的伪狂犬病毒的分析表明，需要持续监测和开发针对伪狂犬病毒特定变体的疫苗。
Since late 2011, outbreaks of pseudorabies virus (PRV) have occurred in southern China causing major economic losses to the pig industry. We previously reported that variant PRV forms and recombination in China could be the source of continued epidemics. Here, we analyzed samples from intensive pig farms in eastern China between 2017 and 2019, and sequenced the main glycoproteins (gB, gC, gD, and gE) to study the evolution characteristics of PRV. Based on the gC gene, we found that PRV variants belong to clade 2 and detected a founder effect during by the PRV epidemic. In addition, we detected inter- and intra-clade recombination; in particular, inter-clade recombination in the gB genes of strains FJ-ZXF and FJ-W2, which were recombinant with clade 1 strains. We also found specific amino-acid changes and positively selected sites, possibly associated with functional changes. This analysis of the emergence of PRV in China illustrates the need for continuous monitoring and the development of vaccines against specific variants of PRV.