EFFECTS OF HYPOXIA ON TYROSINE HYDROXYLASE ACTIVITY IN RAT CAROTID BODY
EFFECTS OF HYPOXIA ON TYROSINE HYDROXYLASE ACTIVITY IN RAT CAROTID BODY
复制标题
缺氧对大鼠颈动脉体酪氨酸羟化酶活性的影响
DOI：
10.1111/j.1471-4159.1979.tb05216.x
复制
发表时间：
1979
影响因子：
4.7
通讯作者：
S. Fidone
中科院分区：
文献类型：
--
作者：
Constancio González;Y. Kwok;J. Gibb;S. Fidone
摘要-酪氨酸羟化酶（TH）活性测定在颈动脉体。正常条件下以及动物在低氧环境中暴露1 - 3 h后48 h时大鼠的上级颈神经节和肾上腺。颈动脉体和神经节的基础TH水平均为5 - 6 nmol/h/mg组织。缺氧后48小时，两种组织中的酶活性均增加，这表明缺氧的严重程度，但颈动脉体中的酶活性高于上级颈神经节。因此，在暴露于N2中的5%O2两个30分钟的时间段（20分钟的间歇期）后，颈动脉体中的TH活性增加了50%，神经节中增加了33%;在暴露于N2中的10%O2 3小时（连续）后，颈动脉体中的TH水平增加了37%，神经节中增加了12%。在肾上腺中，基础TH活性为3.42 ± 1.87 nmol/h/mg组织，并且该值在任一缺氧水平下均不变。
Abstract— Tyrosine hydroxylase (TH) activity was measured in the carotid body. superior cervical ganglion and adrenal glands of the rat under normal conditions and at 48 h following exposure of the animals for 1‐3 h in a low O2 atmosphere. Basal TH levels were 5‐6 nmol/h/mg tissue for both the carotid body and the ganglion. Forty‐eight hours after hypoxia, there was an increase in enzyme activity in both tissues which paralleled the severity of the hypoxia but was greater in the carotid body than the superior cervical ganglion. Thus, following exposure to 5% O2 in N2 for two 30‐min periods (20‐min interim), TH activity had increased by 50% in the carotid body and 33% in the ganglion; after exposure to 10% O2 in N2 for 3 h (continuous), TH levels were increased by 37% in the carotid body and 12% in the ganglion. In the adrenal gland, basal TH activity was 3.42 ± 1.87 nmol/h/mg tissue, and this value was unchanged following either level of hypoxia.