Effects of moderate exercise and oat β-glucan on lung tumor metastases and macrophage antitumor cytotoxicity
Effects of moderate exercise and oat β-glucan on lung tumor metastases and macrophage antitumor cytotoxicity
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DOI：
10.1152/japplphysiol.00252.2004
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发表时间：
2004-09-01
影响因子：
3.3
通讯作者：
Ghaffar, A
中科院分区：
文献类型：
--
作者：
Murphy, EA;Davis, JM;Ghaffar, A
适度的运动和可溶性纤维β-葡聚糖都可以对肿瘤的发生和生长产生有益的影响，但数据有限，并且没有关于其联合作用的信息。本研究测试了短期适度运动训练和可溶性燕麦纤维β-葡聚糖（ObetaG）对注射肿瘤细胞转移扩散和巨噬细胞抗肿瘤细胞毒性的独立和联合作用。将雄性C57 BL/6小鼠分配到四个组中的一个：运动（Ex）-H2O、Ex-ObetaG、对照（Con）-H2O或Con-ObetaG。在肿瘤施用和死亡之前，在饮用水中喂养ObetaG 10天。运动包括跑步机运行（1小时/天）6天。在训练的最后一天休息或运动后，通过静脉注射给予同源B16黑素瘤细胞（2 × 10（5））（每组n = 8-11）。14天后取出肺，并对肿瘤病灶进行计数。杀死另外的小鼠（每组n = 8），并测定腹膜巨噬细胞在各种效应物与靶标比率下对相同小鼠肿瘤细胞系的细胞毒性。适度运动和ObetaG均减少肺肿瘤病灶并增加巨噬细胞细胞毒性。然而，Ex-ObetaG与Ex-H2O或Con-ObetaG之间的肺肿瘤病灶和巨噬细胞细胞毒性没有差异。这些数据表明，尽管短期适度运动训练和食用可溶性ObetaG的作用不是相加的，但它们都可以减少注射的B16黑色素瘤细胞的转移扩散，并且这些作用可能部分是通过增加巨噬细胞的细胞毒性来介导的。B16黑色素瘤。
Both moderate exercise and the soluble fiber beta-glucan can have beneficial effects on the initiation and growth of tumors, but the data are limited, and there is no information on their combined effects. This study tested the independent and combined effects of short-term moderate-exercise training and the soluble oat fiber beta-glucan (ObetaG) on the metatastic spread of injected tumor cells and macrophage antitumor cytotoxicity. Male C57BL/6 mice were assigned to one of four groups: exercise (Ex)-H2O, Ex-ObetaG, control (Con)-H2O, or Con-ObetaG. ObetaG was fed in the drinking water for 10 days before tumor administration and death. Exercise consisted of treadmill running (1 h/day) for 6 days. After rest or exercise on the last day of training, syngeneic B16 melanoma cells (2 x 10(5)) were administered via intravenous injection (n = 8-11 per group). Lungs were removed 14 days later, and tumor foci were counted. Additional mice (n = 8 per group) were killed, and peritoneal macrophages were assayed for cytotoxicity against the same mouse tumor cell line at various effector-to-target ratios. Both moderate exercise and ObetaG decreased lung tumor foci and increased macrophage cytotoxicity. However, there were no differences in lung tumor foci and macrophage cytotoxicity between Ex-ObetaG and either Ex-H2O or Con-ObetaG. These data suggest that, although not additive in their effects, both short-term moderate-exercise training and consumption of the soluble ObetaG can decrease the metatastic spread of injected B16 melanoma cells, and these effects may be mediated in part by an increase in macrophage cytotoxicity to B16 melanoma.