Analysis of Photosystem I Donor and Acceptor Sides with a New Type of Online-Deconvoluting Kinetic LED-Array Spectrophotometer.
Analysis of Photosystem I Donor and Acceptor Sides with a New Type of Online-Deconvoluting Kinetic LED-Array Spectrophotometer.
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使用新型在线解卷积动力学 LED 阵列分光光度计分析光系统 I 供体和受体侧。
DOI：
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发表时间：
2016
影响因子：
4.9
通讯作者：
C. Klughammer
中科院分区：
文献类型：
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作者：
U. Schreiber;C. Klughammer
利用新近开发的Dual/KLAS-NIR分光光度计测定了常春藤、红豆杉和甘蓝型油菜完整叶片中P700、质体蓝素（PC）和铁氧还蛋白（Fd）的氧化还原变化。PC+和Fd-信号，展示了丰富的新的信息和所获得的结果的一致性。在暗适应之后，Fd-变化特别大。在暗光诱导和稳态光响应曲线中，PC氧化先于P700氧化。诱导过程中Fd再氧化与同时测量的荧光产量的二次下降相关，这两者都通过去除O2来消除。通过测定100%氧化还原变化，可以评估PC/P700和Fd/P700的相对含量，其在不同叶片之间显示出相当大的变化，在阳光叶片中具有较高值的趋势。基于去卷积的P700+信号，PSI，Y（I）的互补量子产率（光化学能使用），Y（ND）（由于氧化的主要供体导致的非光化学损失）和Y（NA）（非光化学损失，由于减少受体）被确定为光强度的函数，并与相应的互补量子产率PSII，Y（II）进行比较，（光化学能量使用）、Y（NPQ）（受管制的非光化学损失）和Y（NO）（非管制的非光化学损失）。比值Y（I）/Y（II）随着强度的增加而增加。在低强度范围内，PC+的两步增加指示异质性PC池。
The newly developed Dual/KLAS-NIR spectrophotometer, technical details of which were reported very recently, is used in measuring redox changes of P700, plastocyanin (PC) and ferredoxin (Fd) in intact leaves of Hedera helix, Taxus baccata and Brassica napus An overview of various light-/dark-induced changes of deconvoluted P700+, PC+ and Fd- signals is presented demonstrating the wealth of novel information and the consistency of the obtained results. Fd- changes are particularly large after dark adaptation. PC oxidation precedes P700 oxidation during dark-light induction and in steady-state light response curves. Fd reoxidation during induction correlates with the secondary decline of simultaneously measured fluorescence yield, both of which are eliminated by removal of O2 By determination of 100% redox changes, relative contents of PC/P700 and Fd/P700 can be assessed, which show considerable variations between different leaves, with a trend to higher values in sun leaves. Based on deconvoluted P700+ signals, the complementary quantum yields of PSI, Y(I) (photochemical energy use), Y(ND) (non-photochemical loss due to oxidized primary donor) and Y(NA) (non-photochemical loss due to reduced acceptor) are determined as a function of light intensity and compared with the corresponding complementary quantum yields of PSII, Y(II) (photochemical energy use), Y(NPQ) (regulated non-photochemical loss) and Y(NO) (non-regulated non-photochemical loss). The ratio Y(I)/Y(II) increases with increasing intensities. In the low intensity range, a two-step increase of PC+ is indicative of heterogeneous PC pools.