Enzymatic Pre-Hydrolysis of high fat Content Dairy Wastewater as a Pretreatment for Anaerobic Digestion
Enzymatic Pre-Hydrolysis of high fat Content Dairy Wastewater as a Pretreatment for Anaerobic Digestion
复制标题
高脂肪乳制品废水的酶预水解作为厌氧消化的预处理
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
T. Amani
中科院分区：
文献类型：
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作者：
E. Mobarak;R. Kasra;M. Nosrati;T. Amani
铜绿假单胞菌 KM110 的酶提取物制剂，保藏号：
采用具有脂肪酶活性（0.3 U/ml）的HQ730879对总脂肪含量为1000 mg/L的合成乳品废水进行酶解预处理。预处理优化为 48 小时水解时间，在 45ae% C 下使用 10% v/v 酶提取物。使用间歇式生物反应器研究了合成乳品废水的生物处理。原废水和预水解废水均在间歇式生物反应器中消化。与原废水相比，厌氧消化效率得到提高（化学需氧量 (COD)、去除效率分别为 90% 和 66%，13 天后沼气产量为 4710 毫升和 2330 毫升）。本研究获得的结果表明，应用预处理工艺水解和溶解脂肪可以改善脂肪废水的生物降解，加速脂肪废水的生物降解。
厌氧消化的过程和减少时间。此外，对多种来源的废水进行预处理是脂肪酶的一个新的且有前景的应用。
Enzymatic extract preparation from Pseudomonas aeruginosa KM110 under accession No.
HQ730879 with lipase activity (0.3 U/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/L total fat content. The pretreatment was optimized for 48 h hydrolysis time, at 45ae% C with 10% v/v enzymatic extract. The biological treatment of synthetic dairy wastewater was investigated using a batch bioreactor. Both raw and prehydrolyzed wastewater was digested in a batch bioreactor. Enhanced anaerobic digestion efficiency compare to raw wastewater was achieved (chemical oxygen demand (COD), removal efficiency of 90% vs. 66% and biogas production of 4710 ml vs. 2330 ml after 13days). The results obtained in this study illustrated that the application of a pretreatment process to hydrolyze and dissolve fats may improve the biological degradation of fatty wastewaters, accelerating
the process and reducing time of anaerobic digestion. Moreover, the pretreatment of wastewater from several sources is a new and promising application for lipases.