Passive Immunization with LINGO-1 Polyclonal Antiserum Afforded Neuroprotection and Promoted Functional Recovery in a Rat Model of Spinal Cord Injury
Passive Immunization with LINGO-1 Polyclonal Antiserum Afforded Neuroprotection and Promoted Functional Recovery in a Rat Model of Spinal Cord Injury
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DOI：
10.1159/000290043
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Zeng, Yan-jun
中科院分区：
文献类型：
--
作者：
Lv, Jun;Xu, Ru-xiang;Zeng, Yan-jun
Lingo-1(富含亮氨酸重复序列和Ig结构域的Nogo受体相互作用蛋白)是参与RhoA激活和轴突再生的NGR受体复合体的重要组成部分。作者报道了用LINGO-1多克隆抗血清被动免疫克服脊髓损伤(SCI)后NGR介导的生长抑制的治疗方法。鞘内注射高滴度兔源性抗血清可在较宽的时间窗口内在损伤部位周围检测到；它在体内阻断LINGO-1，具有高分子特异性。在该动物模型中，LINGO-1抗血清被动免疫显著降低了RhoA的活性，增加了神经元的存活率。以这种方式免疫的成年大鼠在脊髓背侧半横断后显示出某些后肢运动功能的恢复。因此，使用LINGO-1多克隆抗血清进行被动免疫治疗可能是一种有前景的急性脊髓损伤修复策略。版权所有(C)2010 S.Karger AG，巴塞尔
LINGO-1 (leucine-rich repeat and Ig domain-containing, Nogo receptor-interacting protein) is an important component of the NgR receptor complex involved in RhoA activation and axon regeneration. The authors report on passive immunization with LINGO-1 polyclonal antiserum, a therapeutic approach to overcome NgR-mediated growth inhibition after spinal cord injury (SCI). The intrathecally administered high-titer rabbit-derived antiserum can be detected around the injury site within a wide time window; it blocks LINGO-1 in vivo with high molecular specificity. In this animal model, passive immunization with LINGO-1 antiserum significantly decreased RhoA activation and increased neuronal survival. Adult rats immunized in this manner show recovery of certain hindlimb motor functions after dorsal hemisection of the spinal cord. Thus, passive immunotherapy with LINGO-1 polyclonal antiserum may represent a promising repair strategy following acute SCI. Copyright (C) 2010 S. Karger AG, Basel