Glycoprotein E-rns of pestiviruses induces apoptosis in lymphocytes of several species
Glycoprotein E-rns of pestiviruses induces apoptosis in lymphocytes of several species
复制标题
DOI：
10.1128/jvi.71.9.6692-6696.1997
复制
发表时间：
1997-09-01
影响因子：
5.4
通讯作者：
vanOirschot, JT
中科院分区：
文献类型：
--
作者：
Bruschke, CJM;Hulst, MM;vanOirschot, JT
猪瘟病毒和牛病毒性腹泻病毒都是瘟病毒属的成员，属于黄病毒科，最近，包膜糖蛋白E-rns被鉴定为一种RNA酶，RNA酶可以表达不同的生物学作用，它们已被证明具有神经毒性、抗蠕虫和免疫抑制作用。我们在体外研究了E-rns的免疫抑制特性，该糖蛋白完全抑制了刀豆球蛋白A诱导的猪、牛、羊和人淋巴细胞的增殖，然后我们在蛋白质合成实验中研究了E-rns对淋巴细胞和上皮细胞的直接细胞毒性作用。 E-rns强烈抑制不同物种淋巴细胞的蛋白质合成，且不损伤细胞膜，这表明存在凋亡过程，并且与E-rns孵育后确实检测到淋巴细胞凋亡，瘟病毒感染的特征是白细胞减少和免疫抑制，我们的结果表明E-rns在瘟病毒的发病机制中发挥重要作用。
Classical swine fever virus and bovine virus diarrhea virus are members of the genus pestivirus, which belongs to the family of the Flaviviridae, Recently, envelope glycoprotein E-rns was identified as an RNase, RNases can express different biological actions, They have been shown to be neurotoxic, antihelminthic, and immunosuppressive. We studied the immunosuppressive properties of E-rns in vitro, The glycoprotein totally inhibited concanavalin A induced proliferation of porcine, bovine, ovine, and human lymphocytes, We then studied the direct cytotoxic effects of E-rns on lymphocytes and epithelial cells in protein synthesis assays. E-rns strongly inhibited the protein synthesis of lymphocytes of different species, without cell membrane damage, This suggested an apoptotic process, and indeed apoptosis of lymphocytes was detected after incubation with E-rns, Pestivirus infections are characterized by leukopenia and immunosuppression, Our results suggest that E-rns plays an important role in the pathogenesis of pestiviruses.