Gene expression analysis of in vitro cocultures to study interactions between breast epithelium and stroma.
Gene expression analysis of in vitro cocultures to study interactions between breast epithelium and stroma.
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DOI：
10.1155/2011/520987
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发表时间：
2011
影响因子：
--
通讯作者：
Troester MA
中科院分区：
文献类型：
--
作者：
Casbas-Hernandez P;Fleming JM;Troester MA
乳腺上皮和间质之间的相互作用是正常组织稳态和肿瘤发生和发展的基础。体外共培养模型的基因表达研究表明，体外模型与体内肿瘤进展相关。例如，基质基因表达已被证明与体内肿瘤亚型相关，并且类似的体外共培养物概括了亚型特异性生物学相互作用。共培养可用于研究癌细胞与特定基质组分（例如，免疫细胞，成纤维细胞，内皮）和不同的代表性细胞系（例如，癌症相关的成纤维细胞与正常相关的成纤维细胞与已建立的永生化成纤维细胞）可以帮助阐明基质变异在肿瘤表型中的作用。基因表达数据也可以与基于细胞的测定相结合，以鉴定与基因表达变化相关的细胞表型。共培养系统是可操作的系统，其可以产生关于细胞-细胞相互作用以及随着肿瘤和间质共同进化而发生的细胞表型的重要见解。
The interactions between breast epithelium and stroma are fundamental to normal tissue homeostasis and for tumor initiation and progression. Gene expression studies of in vitro coculture models demonstrate that in vitro models have relevance for tumor progression in vivo. For example, stromal gene expression has been shown to vary in association with tumor subtype in vivo, and analogous in vitro cocultures recapitulate subtype-specific biological interactions. Cocultures can be used to study cancer cell interactions with specific stromal components (e.g., immune cells, fibroblasts, endothelium) and different representative cell lines (e.g., cancer-associated versus normal-associated fibroblasts versus established, immortalized fibroblasts) can help elucidate the role of stromal variation in tumor phenotypes. Gene expression data can also be combined with cell-based assays to identify cellular phenotypes associated with gene expression changes. Coculture systems are manipulable systems that can yield important insights about cell-cell interactions and the cellular phenotypes that occur as tumor and stroma co-evolve.
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DOI：
10.1158/1078-0432.ccr-08-1283
发表时间：
2009-02-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Beck AH;Espinosa I;Edris B;Li R;Montgomery K;Zhu S;Varma S;Marinelli RJ;van de Rijn M;West RB
通讯作者：
West RB
影响因子：
4.8
作者：
Buess, Martin;Rajski, Michal;Rochlitz, Christoph
通讯作者：
Rochlitz, Christoph
影响因子：
5
作者：
Beck, Andrew H.;Espinosa, Inigo;West, Robert B.
通讯作者：
West, Robert B.
影响因子：
--
作者：
Costa Santos, Rosangela Portilho;Benvenuti, Ticiana Thomazine;Azevedo Koike Folgueira, Maria Aparecida
通讯作者：
Azevedo Koike Folgueira, Maria Aparecida
DOI：
10.1186/bcr2358
发表时间：
2009
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Behbod F;Kittrell FS;LaMarca H;Edwards D;Kerbawy S;Heestand JC;Young E;Mukhopadhyay P;Yeh HW;Allred DC;Hu M;Polyak K;Rosen JM;Medina D
通讯作者：
Medina D