CELLULAR IONS IN HYPERTENSION, DIABETES, AND OBESITY - A NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPIC STUDY
CELLULAR IONS IN HYPERTENSION, DIABETES, AND OBESITY - A NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPIC STUDY
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DOI：
10.1161/01.hyp.17.6.951
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发表时间：
1991-06-01
期刊：
影响因子：
8.3
通讯作者：
LARAGH, JH
中科院分区：
文献类型：
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作者：
RESNICK, LM;GUPTA, RK;LARAGH, JH
为了研究高血压、非胰岛素依赖型糖尿病（NIDDM）和肥胖之间的细胞基础，我们使用P-31和F-19核磁共振波谱法测量了肥胖和NIDDM伴和不伴高血压受试者红细胞内的pH（pH（i））、游离镁（Mg（i））和胞浆游离钙（Ca（i））。 与血压正常的非糖尿病对照组相比（Ca（i）），25.2 +/- 1.4 nM;在正常血压和高血压患者中，Mg（i），232 +/- 8-mu-M），Ca（i）均升高，（36.8 +/- 2.7 nM，sig = 0.005）和高血压（43.4 +/- 2.9 nM，sig = 0.001）NIDDM受试者，Mg（i）同时受到抑制（正常血压：206 +/- 11-μ-M，sig = 0.05;高血压：196 +/- 8-μ-M，sig = 0.001）。 在肥胖的受试者中也观察到了类似但不那么显著的变化。 所有高血压组的pH（i）值均显著低于正常血压对照组（sig = 0.05）。 所有受试者的Ca（i）与舒张压之间均存在连续性关系（r = 0.649，p < 0.001）与体重指数Mg（i）与舒张压之间的相关系数r = 0.564，p < 0.001糖尿病患者pH（i）与舒张压（r =-0.680，p <0.001）相关。 因此，升高的Ca（i）和抑制的Mg（i）和pH（i）水平的星座是高血压状态的特征。 这些异常的细胞离子处理的全部或部分常见的高血压，糖尿病和肥胖症可能有助于这些综合征的病理生理学，并可能有助于解释其频繁的临床共存。
To investigate the cellular basis linking hypertension, non-insulin-dependent diabetes mellitus (NIDDM), and obesity, we used P-31 and F-19 nuclear magnetic resonance spectroscopy to measure intracellular pH (pH(i)), free magnesium (Mg(i)), and cytosolic free calcium (Ca(i)) in erythrocytes of obese and NIDDM subjects with and without hypertension. Compared with normotensive, nondiabetic controls (Ca(i)), 25.2 +/- 1.4 nM; Mg(i), 232 +/- 8-mu-M), Ca(i) was elevated in both normotensive (36.8 +/- 2.7 nM, sig = 0.005) and hypertensive (43.4 +/- 2.9 nM, sig = 0.001) NIDDM subjects, and Mg(i) was concomitantly suppressed (normotensive: 206 +/- 11-mu-M, sig = 0.05; hypertensive: 196 +/- 8-mu-M, sig = 0.001). Similar but less striking changes were noted in obese subjects. Values of pH(i) were significantly lower (sig = 0.05) in all hypertensive groups compared with their normotensive controls. Continuous relations were observed for all subjects between Ca(i) and diastolic blood pressure (r = 0.649, p < 0.001) and body mass index (r = 0.564, p < 0.001), between Mg(i) and diastolic blood pressure (r = - 0.563, p < 0.001) and fasting blood glucose ( r = - 0.580, p < 0.001), and in diabetics, between pH(i) and diastolic blood pressure (r = - 0.680, p < 0.001). Thus, the constellation of elevated Ca(i) and suppressed Mg(i) and pH(i) levels is characteristic of the hypertensive state. These abnormalities of cellular ion handling in whole or in part common to hypertension, diabetes, and obesity may contribute to the pathophysiology of these syndromes and may help to explain their frequent clinical coexistence.