Alterations of plasma membrane fatty acid composition modify the kinetics of Na+ current in cultured rat diencephalic neurons.
Alterations of plasma membrane fatty acid composition modify the kinetics of Na+ current in cultured rat diencephalic neurons.
复制标题
质膜脂肪酸组成的改变改变了培养的大鼠间脑神经元中Na电流的动力学。
DOI：
10.1016/0006-8993(92)90566-r
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Ahmed,Z
中科院分区：
文献类型：
--
作者：
Park,CC;Ahmed,Z
在质膜脂肪酸组成发生改变的游离间脑神经元中，研究了向内Na++电流（INa）的性质。这些神经元生长在补充了w3类（亚麻酸，18:3w3）或w6类（亚油酸，18:2w6）必需脂肪酸（EFA）的培养基中，这导致质膜磷脂多不饱和脂肪酸（PUFA）浓度增加两倍。将这些神经元的内向神经元的特性与在不补充任何脂肪酸的情况下生长的对照神经元的特性进行比较。此外，还研究了添加w9类非必需脂肪酸（油酸，18:1w9）的神经元的免疫功能。有几种性质已被不同程度地修改。快、慢衰减分量的振幅比以及快衰减分量的时间常数随膜磷脂PUFA组成的变化呈一致可逆的变化。电流-电压关系、通道选择性、失活率和失活恢复率没有变化。其他参数，如峰值时间和稳态失活曲线，在补充EFA和nfa的培养中发生了变化，并没有完全逆转。这些发现表明，inaca的动力学可以通过补充脂肪酸来改变。这些影响可以部分地与质膜磷脂脂肪酸组成的改变相关。
Properties of inward Na++currents (INa) were examined in dissociated diencephalic neurons whose plasma membrane fatty acid composition had been altered. These neurons were grown in a defined medium supplemented with essential fatty acids (EFA) of either the w3 class (linolenic acid, 18:3w3) or the w6 class (linoleic acid, 18:2w6), which resulted in a two-fold increase in the plasma membrane phospholipid polyunsaturated fatty acid (PUFA) concentration. The properties of the inward INaof these neurons were compared with those of control neurons grown in the absence of any supplemented fatty acids. The INaof neuronssupplemented with a non-essential fatty acid (NFA) of w9 class (oleic acid, 18:1w9) was also examined. Several properties have been modified to different degrees. The ratio of the amplitudes between the fast and the slow decay components as well as the time constant of the fast decay component changed consistently and reversibly with the membrane phospholipid PUFA composition. The current-voltage relationships, channel selectivity, rates of inactivation and recovery from inactivation did not change. Other parameters, such as time-to-peak and steady-state inactivation curves, have changed in EFA- and NFA-supplemented cultures and did not reverse completely. These findings demonstrate that the kinetics of INacan be modified by fatty acid supplementation. These effects can be correlated, in part, with alterations in plasma membrane phospholipid fatty acid composition.