Essential role of ferrous iron in cyanide‐resistant respiration in Hansenula anomala
Essential role of ferrous iron in cyanide‐resistant respiration in Hansenula anomala
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二价铁在异常汉逊酵母抗氰化物呼吸中的重要作用
DOI：
10.1016/0014-5793(90)80302-y
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
A. Yoshimoto
中科院分区：
文献类型：
--
作者：
N. Minagawa;S. Sakajo;T. Komiyama;A. Yoshimoto
抗霉素a诱导的异常汉草抗氰呼吸完全被邻菲罗啉，α，α′-二吡啶阻断。8 -羟基喹啉。或在抗霉素A和菲罗啉存在的情况下，用[35S]蛋氨酸对细胞进行脉冲标记表明，即使在这些条件下，线粒体中也形成了先前报道的参与抗氰化物呼吸的36-kDa蛋白[(1989)J. Biochem. 105, 864-866]。在环己亚胺存在的情况下，向这些细胞中添加Fe2+而不是Fe3+，导致抗氰化物呼吸活性的快速表达。这些结果表明，在抗霉素A和菲罗啉存在的情况下，36 kda蛋白的非活性形式形成，Fe2+离子将其转化为活性形式。也有可能Fe2+离子参与了抗氰呼吸的反应机制。
Antimycin A-dependent induction of cyanide-resistant respiration inHansenula anomalawas completely blocked byo-phenanthroline, α,α'-dipyridyl. or 8-hydroxyquinoline. Pulse-labeling of the cells with [35S] methionine in the presence of both antimycin A ando-phenanthroline indicated that the 36-kDa protein previously reported to be involved in cyanide-resistant respiration [(1989) J. Biochem. 105, 864-866] was formed in mitochondria even under these conditions. The addition of Fe2+, but not Fe3+, ions to these cells in the presence of cycloheximide resulted in the rapid expression of cyanide-resistant respiration activity. These results suggest that in the presence of both antimycin A ando-phenanthroline an inactive form of the 36-kDa protein was formed and Fe2+ions converted it to the active form. It is also likely that Fe2+ions are involved in the reaction mechanism of cyanide-resistant respiration.
寄生虫布氏锥虫的血流呼吸依赖于类似植物的替代氧化酶。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
ClarksonJr,AB;Bienen,EJ;Pollakis,G;Grady,RW
通讯作者：
Grady,RW