Experimental cannabidiol treatment reduces early pancreatic inflammation in type 1 diabetes
Experimental cannabidiol treatment reduces early pancreatic inflammation in type 1 diabetes
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DOI：
10.3233/ch-168021
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发表时间：
2016-01-01
影响因子：
2.1
通讯作者：
Zhou, Juan
中科院分区：
文献类型：
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作者：
Lehmann, Christian;Fisher, Nicholas B.;Zhou, Juan
背景：1 型糖尿病 (T1D) 中产生胰岛素的 β 细胞的破坏是由引起胰腺炎症的免疫细胞入侵引起的。大麻二酚 (CBD) 是一种植物大麻素，源自大麻植物，被证明可以降低非肥胖糖尿病 (NOD) 小鼠（一种自发性 T1D 发展的动物模型）的糖尿病发病率。 目的：本研究的目的是通过 NOD 小鼠的活体显微镜 (IVM) 来研究实验性 CBD 治疗对 T1D 早期胰腺炎症的影响。 方法：七周大的雌性 NOD 小鼠每天预防性施用 5 mg/kg CBD 或对照载体腹膜内注射。每周五次，持续十周。通过血糖测试确认 T1D 诊断后，对动物进行 IVM。通过 IVM 对白细胞活化和功能性毛细血管密度 (FCD) 进行定量。结果：CBD 治疗的 NOD 小鼠后来发展为 T1D，并显示出白细胞活化显着减少，胰腺微循环中的 FCD 增加。结论：实验性 CBD 治疗减少了通过活体显微镜研究的胰腺微循环中的炎症标志物。
BACKGROUND: Destruction of the insulin-producing beta cells in type 1 diabetes (T1D) is induced by invasion of immune cells causing pancreatic inflammation. Cannabidiol (CBD), a phytocannabinoid, derived from the plant, Cannabis sativa, was shown to lower the incidence of diabetes in non-obese diabetic (NOD) mice, an animal model of spontaneous T1D development.OBJECTIVE: The goal of this study was to investigate the impact of experimental CBD treatment on early pancreatic inflammation in T1D by intravital microscopy (IVM) in NOD mice.METHODS: Seven-week-old female NOD mice were prophylactically administered daily 5 mg/kg CBD or control vehicle i.p. five times weekly for ten weeks. Animals underwent IVM following confirmation of T1D diagnosis by blood glucose testing. Leukocyte activation and functional capillary density (FCD) were quantified via IVM.RESULTS: CBD-treatedNODmice developed T1D later and showed significantly reduced leukocyte activation and increased FCD in the pancreatic microcirculation.CONCLUSIONS: Experimental CBD treatment reduced markers of inflammation in the microcirculation of the pancreas studied by intravital microscopy.