Target-effector interaction in the human and murine natural killer system: specificity and xenogeneic reactivity of the solubilized natural killer-target structure complex and its loss in a somatic cell hybrid.
Target-effector interaction in the human and murine natural killer system: specificity and xenogeneic reactivity of the solubilized natural killer-target structure complex and its loss in a somatic cell hybrid.
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人和鼠类天然杀手级系统中的靶向效应相互作用：溶解的天然杀手靶结构复合物的特异性和异类反应性及其在体细胞杂种中的损失。
DOI：
10.1084/jem.150.3.471
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发表时间：
1979-09-19
期刊：
影响因子：
--
通讯作者：
Jondal M
中科院分区：
文献类型：
--
作者：
Roder JC;Ahrlund-Richter L;Jondal M
预孵育的自然杀伤（NK）细胞与电泳纯化的蛋白质从各种NK敏感的小鼠和人类肿瘤细胞特异性地防止随后的结合到完整的，同源的靶细胞。NK靶结构（NK-TS）由四种特征分子中的部分或全部组成，基于十二烷基硫酸钠-聚丙烯酰胺凝胶中的电泳迁移率，暂定分子量为140 K、160 K、190 K和240 K（+/-10 K）。当在交叉抑制试验中比较这些NK-TS分子时，大的240 K分子最常携带独特的NK特异性，而较小的140 K分子在小鼠中的YAC、136-6和X-63之间以及在人类中的Molt-4和K562之间发生交叉反应。小鼠NK细胞识别与人NK细胞不同的NK-TS分子谱。在NK敏感细胞系（YAC-IR）和不敏感细胞系（A9 HT）之间的克隆体细胞杂交体中部分揭示了NK-TS表达的控制。该杂交体不表达NK-TS，并且不与NK细胞结合，这与先前报道的阴性NK细胞溶解结果一致。尽管多种NK-TS蛋白分子中的一些具有独特的特异性，但交叉反应广泛存在。这些观察结果结合在一起表明NK细胞是多特异性的，并且在识别结构中具有一定的异质性，尽管远低于抗体结合位点的预期。
Preincubation of natural killer (NK) cells with electrophoresis purified proteins from a variety of NK-sensitive murine and human tumor cells specifically prevented subsequent binding to the intact, homologous target cell. The NK-target structures (NK-TS) consisted of some or all of four characteristic molecular species, tentatively assigned molecular weights of 140K, 160K, 190K, and 240K (+/-10K) based on electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels. When these NK-TS molecules were compared in cross-inhibition assays, the large 240K molecule most often carried the unique NK specificity, whereas the smaller 140K molecules cross-reacted between YAC, 136-6 and X-63 in the mouse and between Molt-4 and K562 in the human. Mouse NK cells recognised a different spectrum of NK-TS molecules than human NK cells. The control of NK-TS expression was partially revealed in a cloned, somatic cell hybrid bwtween an NK sensitive (YAC-IR) and insensitive (A9HT) cell line. The hybrid did not express NK-TS and did not bind to NK cells which is in accordance with negative NK cytolytic results previously reported. Although unique specificities are carried by some of the multiple NK-TS protein molecules, cross-reactions were widespread. These observations taken together suggest that the NK cell is polyspecific and has some heterogeneity in the recognition structure although much less than would be expected of an antibody-combining site.