Femtosecond Laser-Assisted Corneal Small Incision Allogenic Intrastromal Lenticule Implantation in Monkeys: A Pilot Study
Femtosecond Laser-Assisted Corneal Small Incision Allogenic Intrastromal Lenticule Implantation in Monkeys: A Pilot Study
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DOI：
10.1167/iovs.14-15296
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发表时间：
2015-06-01
影响因子：
4.4
通讯作者：
Zhou, Xingtao
中科院分区：
文献类型：
--
作者：
Liu, Rui;Zhao, Jing;Zhou, Xingtao
目的.晶状体植入术可用于矫正视力问题。然而，它受到自体微透镜来源的显着抑制。本研究旨在探讨飞秒激光辅助下小切口角膜基质内异体义眼台植入术（AILI）的可行性和疗效。本研究包括6只健康成年猴。在两只猴子的一只眼睛和一只猴子的两只眼睛中进行飞秒瞳孔提取（-4.0屈光度[D]校正，5.0-mm光学区）。将每个提取的屈光小块同种异体移植到另外两只猴子的一只眼睛和一只猴子的两只眼睛中的飞秒激光创建的角膜基质袋中。术前和术后（1天或3天，1个月和6个月）进行裂隙灯显微镜检查，角膜地形图，眼前节光学相干断层扫描，并在体内共焦显微镜。角膜水肿发生在术后早期，边界周围有大量的高反射颗粒。角膜组织水肿逐渐减轻。6个月时，神经纤维再生可在神经鞘层中检测到。总体而言，6个月时角膜屈光力增加了3.27 +/- 1.2 D，占预期矫正的82%。在同一时间点，角膜基质比术前厚69 +/- 11 μ m，大致等于植入角膜的最大厚度。无明显并发症发生。AILI技术对于增加角膜基质厚度和改变角膜屈光力似乎是可行且安全的，这可能为治疗角膜扩张、老视和远视提供一种有用的方法。
PURPOSE. Lenticule implantation can be used to correct vision problems. However, it is significantly restrained by the sources of autologous lenticules. The aim of the present study was to investigate the feasibility and effects of femtosecond laser-assisted corneal small incision allogenic intrastromal lenticule implantation (AILI) in monkeys.METHODS. Six healthy adult monkeys were included in this study. Femtosecond lenticule extraction (-4.0 diopter [D] correction, 5.0-mm optical zone) was performed in one eye of two monkeys and both eyes of one monkey. Each extracted refractive lenticule was allogenically transplanted into a femtosecond laser-created corneal stromal pocket in one eye of the other two monkeys and one monkey's both eyes. Pre-and postoperative (1 or 3 days, 1 month, and 6 months) slit lamp microscopy, corneal topography, anterior segment optical coherence tomography, and in vivo confocal microscopy were performed.RESULTS. Corneal edema occurred in the early postoperative days with a large number of hyperreflective particles around the borders. Corneal tissue edema gradually decreased. Nerve fiber regeneration could be detected in the lenticule layer at 6 months. Overall, 3.27 +/- 1.2 D corneal power was increased at 6 months, accounting for 82% of the intended correction. At the same time point, corneal stroma was 69 +/- 11 mu m thicker than preoperative ones and was roughly equal to the maximum thickness of implanted lenticules. No significant complications were observed.CONCLUSIONS. The AILI technique seems to be feasible and safe for increasing corneal stromal thickness and changing corneal refractive power, which may provide a useful method for treatment of keratoectasia, presbyopia, and hyperopia.