PANDER binds to the liver cell membrane and inhibits insulin signaling in HepG2 cells
PANDER binds to the liver cell membrane and inhibits insulin signaling in HepG2 cells
复制标题
PANDER 与肝细胞膜结合并抑制 HepG2 细胞中的胰岛素信号传导。
DOI：
10.1016/j.febslet.2009.08.008
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发表时间：
2009-09-17
期刊：
影响因子：
3.5
通讯作者：
Wolf, Bryan A.
中科院分区：
文献类型：
--
作者：
Yang, Jichun;Wang, Chunjiong;Wolf, Bryan A.
PANDER是一种与胰岛β细胞胰岛素共分泌的细胞因子。到目前为止，PANDER的生理功能在很大程度上仍然未知。在这里，我们表明，PANDER结合肝膜（125）I-PANDER饱和和竞争性结合试验。在HepG 2细胞中，PANDER预处理范围从4 pM到4 nM 8 h，导致胰岛素刺激的胰岛素受体和胰岛素受体底物1的激活的最大抑制分别为52%和63%。此外，PANDER治疗还使胰岛素刺激的PI 3 K和pAkt水平分别降低了55%和48%。总之，我们已经确定肝脏是PANDER的新靶点，PANDER可能通过调节肝脏胰岛素信号通路参与糖尿病的进展。(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
PANDER is a cytokine co-secreted with insulin from islet beta-cells. To date, the physiological function of PANDER remains largely unknown. Here we show that PANDER binds to the liver membrane by (125)I-PANDER saturation and competitive binding assays. In HepG2 cells, pre-treatment with PANDER ranging from 4 pM to 4 nM for 8 h resulted in a maximal inhibition of insulin-stimulated activation of insulin receptor and insulin receptor substrate 1 by 52% and 63%, respectively. Moreover, PANDER treatment also reduced insulin-stimulated PI3K and pAkt levels by 55% and 48%, respectively. In summary, we have identified the liver as a novel target for PANDER, and PANDER may be involved in the progression of diabetes by regulating hepatic insulin signaling pathways. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.