A new "miniSTR-multiplex" displaying reduced amplicon lengths for the analysis of degraded DNA
A new "miniSTR-multiplex" displaying reduced amplicon lengths for the analysis of degraded DNA
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DOI：
10.1007/s00414-005-0013-6
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发表时间：
2006-03-01
影响因子：
2.1
通讯作者：
Parson, W
中科院分区：
文献类型：
--
作者：
Grubwieser, P;Mühlmann, R;Parson, W
使用重新设计的引物设计了D2S1338、D16S539、D18S51、TH01和FGA位点的多重PCR，以便与市购多重PCR试剂盒（包括amelogenin）的设计相比，缩短扩增产物的长度。新的PCR引物用于从案例样本中扩增高度降解的DNA，这些样本在以前的标准引物组分析中没有或只有较差的结果。这种新型的微型探针的应用提高了降解DNA样品的总体分型成功率。在常规引物与新设计引物的一致性研究中，124个随机选择的个体没有发现基因型差异。
A multiplex PCR was designed for the loci D2S1338, D16S539, D18S51, TH01 and FGA using redesigned primers in order to reduce the lengths of the amplification products compared to the designs used in commercially available multiplex PCR kits, also including amelogenin. The new PCR primers were used to amplify highly degraded DNA from casework samples, which had shown no or only poor results for these loci in previous analyses with standard primer sets. The application of the new miniSTR-multiplex resulted in an increased overall typing success rate for degraded DNA samples. In a concordance study between the conventional and the newly designed primers, no genotype differences were revealed in 124 randomly selected individuals.