Implication of metabolomic profiles to wide thermoneutral zone in Mongolian gerbils (Meriones unguiculatus)
Implication of metabolomic profiles to wide thermoneutral zone in Mongolian gerbils (Meriones unguiculatus)
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代谢组学特征对蒙古沙鼠（Meriones unguiculatus）宽热中性区的影响
DOI：
10.1111/1749-4877.12179
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发表时间：
2016
影响因子：
3.3
通讯作者：
Wang Dehua
中科院分区：
文献类型：
--
作者：
Shi Yaolong;Wang Dehua
长爪沙鼠（Meriones unguiculatus）进化出一个宽的热中性区（26.5-38.9 °C）和高的临界温度上限，似乎对热暴露具有很高的耐受性。在这里，我们使用代谢组学方法来测量沙鼠在较低（27 °C）和较高临界温度（38 °C）之间的总体代谢产物谱，以研究代谢组学特征在维持基础代谢率中的作用。我们发现，在血清和肝脏中，分别有14和19种代谢物发生了显著变化。在有氧呼吸相关三羧酸循环（TCA）中，38 °C动物血清中的5种中间产物（异柠檬酸、顺乌头酸、α-酮戊二酸、富马酸和苹果酸）增加;然而，在肝脏中未发现此类增加。肝脏TCA循环中间体的稳定水平可能与38 °C下有氧呼吸的稳态有关。代谢组学结果还表明，急性热暴露引起蒙古沙鼠的氧化应激和低分子量抗氧化剂的增加。甲硫氨酸和2-羟基丁酸的增加表明谷胱甘肽的合成加速。在38 °C下检测到尿酸盐及其前体肌苷和次黄嘌呤增加。在38 °C下，血清中参与抗坏血酸合成和降解的葡萄糖醛酸、苏糖酸和草酸增加。总之，尽管发现了显著的代谢组学变化，但稳定的肝脏TCA循环可能有助于蒙古沙鼠在宽的热中性区内维持恒定的基础代谢率。
Mongolian gerbils (Meriones unguiculatus) have evolved a wide thermoneutral zone (26.5–38.9 °C) and high upper critical temperature, and appear to have a high tolerance for heat exposure. Here, we use a metabolomic approach to measure global metabolite profiles for gerbils between lower (27 °C) and upper critical temperatures (38 °C) to investigate the role of metabolomic characterization in maintaining basal metabolic rates within a wide thermoneutral zone. We found that in serum and liver, 14 and 19 metabolites were significantly altered, respectively. In the aerobic respiration‐related tricarboxylic cycle (TCA), 5 intermediates (isocitric acid, cis‐aconitic acid, α‐ketoglutaric acid, fumaric acid and malic acid) were increased in serum in 38 °C animals; however, no such increase was found in the liver. A stable level of hepatic TCA cycle intermediates may be related to the steady state of aerobic respiration at 38 °C. Metabolomic results also revealed that acute heat exposure caused increased oxidative stress and low molecular weight antioxidants in Mongolian gerbils. Increased methionine and 2‐hydroxybutyrate suggest an accelerated synthesis of glutathione. Increased urate and its precursors, inosine and hypoxanthine, were detected at 38 °C. Glucuronate, threonate and oxalate involved in ascorbate synthesis and degradation were increased in serum at 38 °C. In conclusion, although dramatic metabolomic variation was found, a stable hepatic TCA cycle may contribute to maintaining a constant basal metabolic rate within a wide thermoneutral zone in Mongolian gerbils.