PHORBOL ESTERS INCREASE THE AMOUNT OF CA-2+, PHOSPHOLIPID-DEPENDENT PROTEIN-KINASE ASSOCIATED WITH PLASMA-MEMBRANE
PHORBOL ESTERS INCREASE THE AMOUNT OF CA-2+, PHOSPHOLIPID-DEPENDENT PROTEIN-KINASE ASSOCIATED WITH PLASMA-MEMBRANE
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DOI：
10.1038/301621a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
ANDERSON, WB
中科院分区：
文献类型：
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作者：
KRAFT, AS;ANDERSON, WB
虽然佛波酯肿瘤促进剂的生物化学作用机制尚未完全了解，但已知佛波酯与细胞表面的结合导致钙流1，2和磷脂代谢3 -7的快速变化。最近已经描述了一种蛋白激酶活性，其依赖于钙和酸性磷脂，并通过二酰基甘油进一步增强8 -10。以前，我们已经观察到，佛波酯处理的EL 4小鼠胸腺瘤细胞引起胞质钙，磷脂依赖性蛋白激酶（Ca，PL-PK）活性的快速下降，这是通过特定的佛波酯细胞表面受体介导的EL 4细胞11。我们现在表明，治疗壁卵黄囊（PYS-2）细胞与生物活性的12-O-十四烷酰基佛波醇-13-乙酸酯（TPA）引起细胞溶质Ca，PL-PK活性的快速下降，伴随着Ca，PL-PK活性与质膜组分的量的显着增加。这些结果表明，Ca，PL-PK活性与质膜的快速和紧密的关联可能是介导佛波酯的一些作用的早期事件。
Although the biochemical mechanism of action of phorbol ester tumour promoters is not fully understood, it is known that phorbol ester binding to the cell surface causes rapid changes in calcium flux1,2and phospholipid metabolism3–7. A protein kinase activity has recently been described which is dependent on calcium and acidic phospholipids and is further enhanced by diacylglycerol8–10. Previously, we have observed that phorbol ester treatment of EL4 mouse thymoma cells causes a rapid decrease in cytosolic calcium, phospholipid-dependent protein kinase (Ca, PL-PK) activity, which is mediated through the specific phorbol ester cell-surface receptors identified on EL4 cells11. We now show that treatment of parietal yolk sacs (PYS-2) cells with biologically active 12-O-tetradecanoyl phorbol-13-acetate (TPA) provokes a rapid decrease in cytosolic Ca, PL-PK activity that is accompanied by a significant increase in the amount of Ca, PL-PK activity associated with the plasma membrane fraction. These results suggest that the rapid and tight association of Ca, PL-PK activity with the plasma membrane may be an early event in mediating some of the effects of phorbol esters.