Studies on culture condition of new marine bacterium Zooshikella sp. SY01
Studies on culture condition of new marine bacterium Zooshikella sp. SY01
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DOI：
10.1007/s11705-008-0065-5
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发表时间：
2008-09
期刊：
影响因子：
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通讯作者：
Wen-Jian Lan;L. Mo;Chuanghua Cai;Yipin Zhou;Junhua Yao;Hou‐Jin Li
中科院分区：
文献类型：
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作者：
Wen-Jian Lan;L. Mo;Chuanghua Cai;Yipin Zhou;Junhua Yao;Hou‐Jin Li
新海洋细菌Zooshikellasp.从三亚湾海水中分离到灵菌红素产生菌SY 01。对该菌的培养条件进行了研究。SY 01菌株在2216 E培养基中培养，培养基中分别含有色氨酸、组氨酸、乳酸、樟脑、柠檬烯、酪蛋白、二苯胍、香豆素和1，3-二硝基苯。培养5d后，采用正离子电喷雾直接进样质谱法对不同培养液的提取物进行检测。结果表明，色氨酸、组氨酸和酪蛋白对灵菌红素的生物合成没有明显影响。乳酸、樟脑、柠檬烯、二苯胍、香豆素在一定程度上可以抑制细菌生长和灵菌红素的生物合成，减缓培养液变红。而1，3-二硝基苯则完全抑制该菌产生灵菌红素。MS数据表明，在不同的培养基中产生的各种代谢产物具有化学多样性。特别是，在含有柠檬烯的培养基中观察到一系列相对丰度高的高分子量化合物。进一步优化培养条件，可以获得更多新的灵菌红素类似物和先导化合物，实现“一株多化合物”的目标。
New marine bacteriumZooshikellasp. SY01, producer of prodigiosin, was isolated from the seawaters of Sanya Bay. The culture conditions of this bacterium were investigated.Zooshikellasp. SY01 was cultured in 2216E media which contained tryptophan, histidine, lactonic acid, camphor, limonene, casein, diphenyl guanidine, coumarin and 1,3-dinitrobenzene, respectively. After 5 days cultivation, the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode. As the results, tryptophan, histidine and casein didn’t show any observable influences on the biosynthesis of prodigiosin. Lactonic acid, camphor, limonene, diphenyl guanidine, coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a certain extent, slower the culture broth to turn red. However, 1,3-dinitrobenzene inhibited the bacteria to produce prodigiosin completely. MS data suggested that various metabolites with chemodiversity were produced in different culture media. In particular, a series of high-molecular-weight compounds with high relative abundances were observed in the medium containing limonene. To further optimize the culture condition, more new prodigiosin analogues and lead compounds can be obtained and the goal of “one strain-many compounds” can be achieved.