Rapid establishment of introgression lines using cytoplasmic male sterility and a restorer gene in Oryza sativa cv. Nippon bare
Rapid establishment of introgression lines using cytoplasmic male sterility and a restorer gene in Oryza sativa cv. Nippon bare
复制标题
利用水稻细胞质雄性不育和恢复基因快速建立基因渗入系。
DOI：
10.1007/s11032-013-9910-4
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发表时间：
2013
影响因子：
3.1
通讯作者：
Kinya Toriyama and Tetsu Kinoshita^*
中科院分区：
文献类型：
--
作者：
Takayuki Ohnishi^*;Mihoko Yoshino;Kinya Toriyama and Tetsu Kinoshita^*
数量性状位点（QTL）分析极大地增强了我们对水稻（Oryza sativa）复杂性状的理解。与此同时，基因渗入系的发展为阐明复杂的遗传网络和鉴定QTL提供了强大的工具。我们最近开发了一种 Biotron 育种系统，可以在室内快速种植水稻。然而，该系统在水稻标记辅助育种的应用中存在两个相对薄弱的地方：第一，品种间世代时间的差异；第二，杂交产生的种子数量少。为了弥补这些弱点，我们建议利用细胞质雄性不育（CMS）和恢复系（Rf）。日本晴的遗传背景。通过利用日本晴的遗传背景，无论供体水稻植株的遗传背景有何差异，都可以实现 2 个月的水稻世代时间。该 CMS-Rf 系统可提高杂交种子的产量，避免去雄并防止意外杂交。我们的结果表明，这种新方法可以显着加速水稻研究的许多不同方面，特别是在功能基因组学方面。 Biotron育种系统、早花习性和CMS的结合对于筛选与QTL相关的候选基因以及将有用的QTL引入优良品种具有重要价值。
Quantitative trait locus (QTL) analyses have greatly enhanced our understanding of complex traits in rice (Oryza sativa). In parallel, the development of introgression lines has provided a powerful tool for elucidation of complicated genetic networks and identification of QTL. We recently developed a biotron breeding system that allows rapid indoor cultivation of rice plants. The system, however, has two relatively weak points in its application to marker-assisted breeding in rice: first, variation in generation times among cultivars; second, the low number of seeds produced by crosses. To compensate for these weaknesses, we propose utilizing cytoplasmic male sterility (CMS) and restorer (Rf) lines with a cv. Nipponbare genetic background. Through use of the Nipponbare genetic background, rice generation times of 2 months can be achieved regardless of any differences in the genetic background of the donor rice plant. This CMS–Rf system confers a high yield of hybrid seeds, avoids the need for emasculation and precludes accidental crosses. Our results demonstrate that this new methodology can markedly accelerate many different aspects of rice research, especially in functional genomics. The combination of biotron breeding system, early flowering habit and CMS will be of great value for screening candidate genes associated with QTL and for introducing useful QTL into elite cultivars.