Mining for treatment-specific and general changes in target compounds and metabolic fingerprints in response to herbivory and phytohormones in Plantago lanceolata.
Mining for treatment-specific and general changes in target compounds and metabolic fingerprints in response to herbivory and phytohormones in Plantago lanceolata.
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DOI：
10.1111/j.1469-8137.2011.03768.x
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发表时间：
2011-09
期刊：
影响因子：
--
通讯作者：
R. Sutter;C. Müller
中科院分区：
文献类型：
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作者：
R. Sutter;C. Müller
关注目标代谢物的诱导研究可能无法揭示植物在各种挑战后发生的代谢变化。相比之下，代谢指纹图谱是一种强大的工具，可以找到特定处理或一般处理的模式，因此可以用来描述植物在各种挑战后的反应。杉木车前草植物受到机械损伤、特殊食草动物（蚜虫或锯蝇幼虫）、通才食草动物（鳞翅目毛虫）或植物激素（茉莉酸或水杨酸）的侵害。处理3 d后，采用气相色谱-质谱联用（GC-MS）和液相色谱-飞行时间质谱联用（LC-TOF-MS）分析局部和全身叶片的特征目标代谢物（环烯醚萜苷和毛蕊花苷）和代谢指纹图谱。虽然目标代谢物浓度只有微小的变化，但代谢指纹受到了实质性的影响，特别是受到综合和植物激素处理的影响。相比之下，机械损伤和专门的草食造成的变化较少。对多面手的反应与茉莉酸引起的变化部分重叠，但许多额外的峰被上调。此外，茉莉酸和水杨酸共同诱导了许多峰。这两种植物激素令人惊讶的高共同诱导峰表明信号通路调节了一组共同的目标。此外，只有代谢指纹图谱才能揭示草食动物在这些植物激素反应之外诱导其他物种特异性途径。
Induction studies focusing on target metabolites may not reveal metabolic changes occurring in plants after various challenges. By contrast, metabolic fingerprinting can be a powerful tool to find patterns that are either treatment-specific or general and was therefore used to depict plant responses after various challenges. Plants of Plantago lanceolata were challenged by mechanical damage, specialist herbivores (aphids or sawfly larvae), generalist herbivores (Lepidopteran caterpillars) or phytohormones (jasmonic or salicylic acid). After 3 d of treatment, local and systemic leaves were analyzed for characteristic target metabolites (iridoid glucosides and verbascoside) by gas chromatography coupled with mass spectrometry (GC-MS) and for metabolic fingerprints by liquid chromatography coupled with time of flight mass spectrometry (LC-TOF-MS). Whereas only marginal changes in target metabolite concentrations were found, metabolic fingerprints were substantially affected especially by generalist and phytohormone treatments. By contrast, mechanical damage and specialist herbivory caused fewer changes. Responses to generalists partly overlapped with the changes caused by jasmonic acid, but many additional peaks were up-regulated. Furthermore, many peaks were co-induced by jasmonic and salicylic acid. The surprisingly high co-induction of peaks by both phytohormones suggests that the signaling pathways regulate a set of common targets. Furthermore, only metabolic fingerprinting could reveal that herbivores induce additional species-specific pathways beyond these phytohormone responses.