Supramolecular Bioimaging through Signal Amplification by Combining Indicator Displacement Assay with Forster Resonance Energy Transfer
Supramolecular Bioimaging through Signal Amplification by Combining Indicator Displacement Assay with Forster Resonance Energy Transfer
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DOI：
10.1002/anie.202104358
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发表时间：
2021-07-30
影响因子：
16.6
通讯作者：
Guo, Dong-Sheng
中科院分区：
文献类型：
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作者：
Geng, Wen-Chao;Ye, Zhongju;Guo, Dong-Sheng
荧光化学传感器是生命科学和工程领域强大的成像工具。基于超分子化学原理，指示剂置换分析（IDA）为构建和优化化学传感器提供了一种替代方法，具有简单、可调谐和模块化的优点。然而，IDA在生物成像中的应用仍然面临一系列挑战，包括干扰信号、背景噪声和不一致的空间位置。因此，我们在此报告了一种利用大环两亲物作为操作平台的福斯特共振能量转移（FRET）辅助IDA的超分子生物成像策略。通过将 FRET 与 IDA 相结合，解决了 IDA 在生物成像中的局限性。作为概念证明，该研究通过信号放大实现了活细胞中三磷酸腺苷的线粒体靶向成像。除了共价方法之外，这项研究还为生物成像开辟了一条非共价途径，在可调谐性、通用性和简单性方面取得了进步。
Fluorescent chemosensors are powerful imaging tools in the fields of life sciences and engineering. Based on the principle of supramolecular chemistry, indicator displacement assay (IDA) provides an alternative approach for constructing and optimizing chemosensors, which has the advantages of simplicity, tunability, and modularity. However, the application of IDA in bioimaging continues to face a series of challenges, including interfering signals, background noise, and inconsistent spatial location. Accordingly, we herein report a supramolecular bioimaging strategy of Forster resonance energy transfer (FRET)-assisted IDA by employing macrocyclic amphiphiles as the operating platform. By merging FRET with IDA, the limitations of IDA in bioimaging were addressed. As a proof of concept, the study achieved mitochondria-targeted imaging of adenosine triphosphate in live cells with signal amplification. This study opens a non-covalent avenue for bioimaging with advancements in tunability, generality, and simplicity, apart from the covalent approach.