DNA MEASUREMENTS AND PLOIDY DETERMINATION OF DEVELOPMENTAL STAGES IN THE LIFE-CYCLES OF THEILERIA-ANNULATA AND THEILERIA-PARVA
DNA MEASUREMENTS AND PLOIDY DETERMINATION OF DEVELOPMENTAL STAGES IN THE LIFE-CYCLES OF THEILERIA-ANNULATA AND THEILERIA-PARVA
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DOI：
10.1007/bf00932023
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发表时间：
1995-08-01
影响因子：
2
通讯作者：
MORZARIA, S
中科院分区：
文献类型：
--
作者：
GAUER, M;MACKENSTEDT, U;MORZARIA, S
结果表明，在不同发育阶段，环形泰勒虫和T.使用荧光染料Hoechst 33258作为染色染料，通过细胞光度DNA技术测量牛和蜱中的细小病毒。结果表明，子孢子、裂殖子、配子和配子均为单倍体，而多核淋巴细胞内配子为多倍体。T. parva和T.在这些阶段中的annulata。对于这两种泰勒虫，DNA测量显示配子融合发生在最终宿主的肠道中，从而提供了有性生殖的证据。然而，在蜱虫中的两种寄生虫之间观察到差异。而T. parva的合子经历了两步减数分裂，在T.环状的在动子的进一步发育中也可以检测到差异，表明泰勒虫物种的特征不仅仅是一个生活史，这是本属特有的。
The relative DNA levels of different developmental stages of Theileria annulata and T. parva in the cow and the tick were measured by the cytophotometric DNA technique using the fluorochrome Hoechst 33258 as a staining dye. The results revealed that sporozoites, merozoites, gamonts, and gametes were haploid, whereas multinucleated intralymphocytic schizonts were poly ploid. No difference was observed between T. parva and T. annulata in these stages. For both Theileria species, the DNA measurements revealed that fusion of gametes occurred in the gut of the final host, thus providing evidence of sexual reproduction. However, differences were observed between the two parasites in the tick. Whereas T. parva zygotes underwent a two-step meiotic division, a comparable reduction division could not be unequivocally detected in T. annulata. Differences could also be detected in the further development of kinetes, indicating that Theileria species are not characterized by only one life cycle, which is specific for this genus.